产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4303, RRID:AB_2836654.
展开/折叠
PABP5; Poly(A) binding protein 5; Poly(A) binding protein cytoplasmic 5; Polyadenylate binding protein 5;
抗原和靶标
- Q96DU9 PABP5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGSGEPNPAGKKKKYLKAALYVGDLDPDVTEDMLYKKFRPAGPLRFTRICRDPVTRSPLGYGYVNFRFPADAEWALNTMNFDLINGKPFRLMWSQPDDRLRKSGVGNIFIKNLDKSIDNRALFYLFSAFGNILSCKVVCDDNGSKGYAYVHFDSLAAANRAIWHMNGVRLNNRQVYVGRFKFPEERAAEVRTRDRATFTNVFVKNIGDDIDDEKLKELFCEYGPTESVKVIRDASGKSKGFGFVRYETHEAAQKAVLDLHGKSIDGKVLYVGRAQKKIERLAELRRRFERLRLKEKSRPPGVPIYIKNLDETINDEKLKEEFSSFGSISRAKVMMEVGQGKGFGVVCFSSFEEATKAVDEMNGRIVGSKPLHVTLGQARRRC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96DU9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y15 | Phosphorylation | Uniprot | |
| Y35 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| K111 | Acetylation | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| Y147 | Phosphorylation | Uniprot | |
| Y149 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| Y270 | Phosphorylation | Uniprot |
研究背景
Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo (By similarity).
Cytoplasm.
Mitochondrion matrix.
Note: Co-fractionates with mtDNA and co-immunoprecipitates with the mitochondrial poly(A) polymerase.
Expressed in fetal brain and in a range of adult tissues.
研究领域
· Genetic Information Processing > Translation > RNA transport.
· Genetic Information Processing > Translation > mRNA surveillance pathway.
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
限制条款
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