SF3B14 Antibody - #DF4325
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4325, RRID:AB_2836693.
展开/折叠
Ht006; P14; PM14_HUMAN; Pre mRNA branch site protein p14; Pre-mRNA branch site protein p14; SAP14; SF3B 14 kDa subunit; SF3B14; SF3B14a; Splicing factor 3B, 14 kDa subunit;
抗原和靶标
- Q9Y3B4 SF3B6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y3B4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K7 | Sumoylation | Uniprot | |
| K7 | Ubiquitination | Uniprot | |
| K29 | Acetylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| Y61 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| C74 | S-Nitrosylation | Uniprot | |
| C83 | S-Nitrosylation | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K116 | Acetylation | Uniprot | |
| K116 | Ubiquitination | Uniprot |
研究背景
Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.
Nucleus.
Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. Within the SF3B complex interacts directly with SF3B1 Ref.20). The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome.
Belongs to the SF3B6 family.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
限制条款
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