| 产品: | SLU7 抗体 |
| 货号: | DF4330 |
| 描述: | Rabbit polyclonal antibody to SLU7 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 65 KD; 68kD(Calculated). |
| 蛋白号: | O95391 |
| RRID: | AB_2836698 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4330, RRID:AB_2836698.
展开/折叠
9G8; hSlu7; MGC9280; Pre mRNA splicing factor SLU7; Pre-mRNA-splicing factor slu7; SLU 7; slu7; SLU7 splicing factor homolog (S. cerevisiae); SLU7 splicing factor homolog; SLU7_HUMAN; splicing factor; Step II splicing factor SLU7; Zinc knuckle motif containing;
抗原和靶标
- O95391 SLU7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSATVVDAVNAAPLSGSKEMSLEEPKKMTREDWRKKKELEEQRKLGNAPAEVDEEGKDINPHIPQYISSVPWYIDPSKRPTLKHQRPQPEKQKQFSSSGEWYKRGVKENSIITKYRKGACENCGAMTHKKKDCFERPRRVGAKFTGTNIAPDEHVQPQLMFDYDGKRDRWNGYNPEEHMKIVEEYAKVDLAKRTLKAQKLQEELASGKLVEQANSPKHQWGEEEPNSQMEKDHNSEDEDEDKYADDIDMPGQNFDSKRRITVRNLRIREDIAKYLRNLDPNSAYYDPKTRAMRENPYANAGKNPDEVSYAGDNFVRYTGDTISMAQTQLFAWEAYDKGSEVHLQADPTKLELLYKSFKVKKEDFKEQQKESILEKYGGQEHLDAPPAELLLAQTEDYVEYSRHGTVIKGQERAVACSKYEEDVKIHNHTHIWGSYWKEGRWGYKCCHSFFKYSYCTGEAGKEIVNSEECIINEITGEESVKKPQTLMELHQEKLKEEKKKKKKKKKKHRKSSSDSDDEEKKHEKLKKALNAEEARLLHVKETMQIDERKRPYNSMYETREPTEEEMEAYRMKRQRPDDPMASFLGQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95391 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| K44 | Sumoylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| Y102 | Phosphorylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| Y163 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| K187 | Sumoylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| K199 | Sumoylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| Y243 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| K257 | Sumoylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| Y284 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| Y297 | Phosphorylation | Uniprot | |
| K302 | Acetylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| Y354 | Phosphorylation | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| Y397 | Phosphorylation | Uniprot | |
| Y400 | Phosphorylation | Uniprot | |
| K408 | Ubiquitination | Uniprot | |
| K418 | Sumoylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| K482 | Sumoylation | Uniprot | |
| K482 | Ubiquitination | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| K540 | Acetylation | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| S582 | Phosphorylation | Uniprot |
研究背景
Required for pre-mRNA splicing as component of the spliceosome. Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.
Nucleus. Nucleus speckle. Cytoplasm.
Note: Predominantly nuclear. Shuttling between the nucleus and the cytoplasm is regulated by the CCHC-type zinc finger. Upon UV-C stress stimulus, the nuclear concentration of the protein decreases, affecting alternative splicing. Translocates from the nucleus to the cytoplasm after heat shock cell treatment. Accumulates in cytoplasmic vesicle-like organelles after heat shock treatment, which may represent stress granules.
Component of pre-catalytic, catalytic and post-catalytic spliceosomes. Associates with the spliceosome prior to recognition of the 3'-splice site for step II, probably during catalysis of step I.
The CCHC-type zinc finger is required to retain the protein within the nucleus and prevent its shuttle back to the cytoplasm via the CRM1 pathway.
Belongs to the SLU7 family.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
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