产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4351, RRID:AB_2836719.
展开/折叠
PMS2 C terminal like protein; PMS2L; PMS2P13; DNA mismatch repair gene homologue; DNA mismatch repair protein PMS2; H_DJ0042M02.9; HNPCC4; Mismatch repair endonuclease PMS2; Mismatch repair gene PMSL2; MLH4; PMS 2; PMS1 homolog 2 mismatch repair system; PMS1 protein homolog 2; PMS2; PMS2 postmeiotic segregation increased 2; PMS2 postmeiotic segregation increased 2 (S. cerevisiae); PMS2_HUMAN; PMS2CL; PMSL2; Postmeiotic segregation increased, S. cerevisiae, 2;
抗原和靶标
- Q68D20 PMS2L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHAADLEKPMVEKQDQSPSLRTGEEKRDVSISRLREAFSLRHTTENKPHSPKTPEPRRSPLGQKRGMSSSSTSDAISDRGVLRPQKEAVSSSQGPSDPTDRAEVEKDSGHGSTSVDSEGFSIPDTGSHCSSECVASTPGDRGSQEHVDSQEKAPETDDSFSDVDCHSNQEDTGCKFQVLPQPTNLTSPNTKVF
- P54278 PMS2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERAESSSTEPAKAIKPIDRKSVHQICSGQVVLSLSTAVKELVENSLDAGATNIDLKLKDYGVDLIEVSDNGCGVEEENFEGLTLKHHTSKIQEFADLTQVETFGFRGEALSSLCALSDVTISTCHASAKVGTRLMFDHNGKIIQKTPYPRPRGTTVSVQQLFSTLPVRHKEFQRNIKKEYAKMVQVLHAYCIISAGIRVSCTNQLGQGKRQPVVCTGGSPSIKENIGSVFGQKQLQSLIPFVQLPPSDSVCEEYGLSCSDALHNLFYISGFISQCTHGVGRSSTDRQFFFINRRPCDPAKVCRLVNEVYHMYNRHQYPFVVLNISVDSECVDINVTPDKRQILLQEEKLLLAVLKTSLIGMFDSDVNKLNVSQQPLLDVEGNLIKMHAADLEKPMVEKQDQSPSLRTGEEKKDVSISRLREAFSLRHTTENKPHSPKTPEPRRSPLGQKRGMLSSSTSGAISDKGVLRPQKEAVSSSHGPSDPTDRAEVEKDSGHGSTSVDSEGFSIPDTGSHCSSEYAASSPGDRGSQEHVDSQEKAPKTDDSFSDVDCHSNQEDTGCKFRVLPQPTNLATPNTKRFKKEEILSSSDICQKLVNTQDMSASQVDVAVKINKKVVPLDFSMSSLAKRIKQLHHEAQQSEGEQNYRKFRAKICPGENQAAEDELRKEISKTMFAEMEIIGQFNLGFIITKLNEDIFIVDQHATDEKYNFEMLQQHTVLQGQRLIAPQTLNLTAVNEAVLIENLEIFRKNGFDFVIDENAPVTERAKLISLPTSKNWTFGPQDVDELIFMLSDSPGVMCRPSRVKQMFASRACRKSVMIGTALNTSEMKKLITHMGEMDHPWNCPHGRPTMRHIANLGVISQN
翻译修饰 - Q68D20/P54278 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K13 | Ubiquitination | Uniprot | |
| K57 | Sumoylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K210 | Acetylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| S405 | Phosphorylation | Uniprot | |
| K413 | Sumoylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| T439 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| K450 | Acetylation | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| S482 | Phosphorylation | Uniprot | |
| T485 | Phosphorylation | Uniprot | |
| S523 | Phosphorylation | Uniprot | |
| T542 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| T573 | Phosphorylation | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| T597 | Phosphorylation | Uniprot | |
| S601 | Phosphorylation | Uniprot | |
| S603 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| S639 | Phosphorylation | Uniprot | |
| Y645 | Phosphorylation | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| K651 | Sumoylation | Uniprot | |
| K651 | Ubiquitination | Uniprot | |
| K748 | Ubiquitination | Uniprot | |
| K766 | Ubiquitination | Uniprot | |
| T777 | Phosphorylation | Uniprot | |
| S791 | Phosphorylation | Uniprot | |
| S793 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | Uniprot | |
| T824 | Phosphorylation | Uniprot | |
| S825 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S59 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot |
研究背景
Belongs to the DNA mismatch repair MutL/HexB family.
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
Nucleus.
Heterodimer of PMS2 and MLH1 (MutL alpha). Forms a ternary complex with MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3). Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MTMR15/FAN1.
Belongs to the DNA mismatch repair MutL/HexB family.
研究领域
· Genetic Information Processing > Replication and repair > Mismatch repair.
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
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