ZIC1/2/3 Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IHC
Cites

1/4

Western blot analysis of extracts from Mouse testis, using ZIC1/2/3 Antibody at 1/1000 dilution.

AF0340 at 1/100 staining Human esophageal cancer by IHC-P.

AF0340 at 1/100 staining human brain tissue sections by IHC-P.

Figure 2 Expression analysis of ZIC2 protein as determined by immunohistochemistry (200×).

产品:	ZIC1/2/3 抗体
货号:	AF0340
描述:	Rabbit polyclonal antibody to ZIC1/2/3
应用:	WB IHC IF/ICC
反应:	Human, Mouse
预测:	Pig, Zebrafish, Bovine, Horse, Sheep, Chicken, Xenopus
分子量:	51kDa; 48kD,55kD,51kD(Calculated).
蛋白号:	Q15915 \| O95409 \| O60481
RRID:	AB_2833294

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse

预测:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(100%)

克隆:

Polyclonal

特异性:

ZIC1/2/3 Antibody detects endogenous levels of total ZIC1/2/3.

RRID:

AB_2833294
引用格式: Affinity Biosciences Cat# AF0340, RRID:AB_2833294.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Odd paired homolog Drosophila; Zic 1; ZIC; Zic family member 1 (odd-paired Drosophila homolog); Zic family member 1; Zic protein member 1; zic1; ZIC1_HUMAN; Zinc finger protein 201; Zinc finger protein of the cerebellum 1; Zinc finger protein ZIC 1; Zinc finger protein ZIC1; ZNF 201; ZNF201; HPE 5; HPE5; Odd paired homolog Drosophila; Zic 2; Zic family member 2 (odd paired Drosophila homolog); Zic family member 2; ZIC2; ZIC2_HUMAN; Zinc finger protein of the cerebellum 2; Zinc finger protein ZIC 2; Zinc finger protein Zic2; Heterotaxy 1; HTX; HTX1; ZIC 3; Zic family member 3 (odd paired Drosophila homolog heterotaxy 1); Zic family member 3; Zic3; ZIC3_HUMAN; Zinc finger protein 203; Zinc finger protein of the cerebellum 3; Zinc finger protein ZIC 3 (Zinc finger protein of the cerebellum 3); Zinc finger protein ZIC 3; ZNF203;

抗原和靶标

免疫原:

Uniprot:

Q15915(ZIC1_HUMAN) >>Visit HPA database.

O95409(ZIC2_HUMAN) >>Visit HPA database.

O60481(ZIC3_HUMAN) >>Visit HPA database.

基因/基因ID:

ZIC1(7545) | ZIC2(7546) | ZIC3(7547)

表达:

Q15915 ZIC1_HUMAN:

CNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined.

描述:

Q15915; ZIC; Zic family member 1 (odd-paired Drosophila homolog); Zic family member 1 (odd-paired homolog, Drosophila); ZIC1; zinc finger protein of the cerebellum 1; zinc finger protein ZIC 1; ZNF201

序列:

Q15915 ZIC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLDAGPQYPAIGVTTFGASRHHSAGDVAERDVGLGINPFADGMGAFKLNPSSHELASAGQTAFTSQAPGYAAAAALGHHHHPGHVGSYSSAAFNSTRDFLFRNRGFGDAAAAASAQHSLFAASAGGFGGPHGHTDAAGHLLFPGLHEQAAGHASPNVVNGQMRLGFSGDMYPRPEQYGQVTSPRSEHYAAPQLHGYGPMNVNMAAHHGAGAFFRYMRQPIKQELICKWIEPEQLANPKKSCNKTFSTMHELVTHVTVEHVGGPEQSNHICFWEECPREGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFKCEFEGCDRRFANSSDRKKHMHVHTSDKPYLCKMCDKSYTHPSSLRKHMKVHESSSQGSQPSPAASSGYESSTPPTIVSPSTDNPTTSSLSPSSSAVHHTAGHSALSSNFNEWYV

O95409 ZIC2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLDAGPQFPAIGVGSFARHHHHSAAAAAAAAAEMQDRELSLAAAQNGFVDSAAAHMGAFKLNPGAHELSPGQSSAFTSQGPGAYPGSAAAAAAAAALGPHAAHVGSYSGPPFNSTRDFLFRSRGFGDSAPGGGQHGLFGPGAGGLHHAHSDAQGHLLFPGLPEQHGPHGSQNVLNGQMRLGLPGEVFGRSEQYRQVASPRTDPYSAAQLHNQYGPMNMNMGMNMAAAAAHHHHHHHHHPGAFFRYMRQQCIKQELICKWIDPEQLSNPKKSCNKTFSTMHELVTHVSVEHVGGPEQSNHVCFWEECPREGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFQCEFEGCDRRFANSSDRKKHMHVHTSDKPYLCKMCDKSYTHPSSLRKHMKVHESSPQGSESSPAASSGYESSTPPGLVSPSAEPQSSSNLSPAAAAAAAAAAAAAAAVSAVHRGGGSGSGGAGGGSGGGSGSGGGGGGAGGGGGGSSGGGSGTAGGHSGLSSNFNEWYV

O60481 ZIC3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTMLLDGGPQFPGLGVGSFGAPRHHEMPNREPAGMGLNPFGDSTHAAAAAAAAAAFKLSPAAAHDLSSGQSSAFTPQGSGYANALGHHHHHHHHHHHTSQVPSYGGAASAAFNSTREFLFRQRSSGLSEAASGGGQHGLFAGSASSLHAPAGIPEPPSYLLFPGLHEQGAGHPSPTGHVDNNQVHLGLRGELFGRADPYRPVASPRTDPYAAGAQFPNYSPMNMNMGVNVAAHHGPGAFFRYMRQPIKQELSCKWIDEAQLSRPKKSCDRTFSTMHELVTHVTMEHVGGPEQNNHVCYWEECPREGKSFKAKYKLVNHIRVHTGEKPFPCPFPGCGKIFARSENLKIHKRTHTGEKPFKCEFEGCDRRFANSSDRKKHMHVHTSDKPYICKVCDKSYTHPSSLRKHMKVHESQGSDSSPAASSGYESSTPPAIASANSKDTTKTPSAVQTSTSHNPGLPPNFNEWYV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Zebrafish

100

Chicken

100

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15915/O95409/O60481 作为底物

Site	PTM Type	Source
Y9	Phosphorylation	Uniprot
T15	Phosphorylation	Uniprot
S20	Phosphorylation	Uniprot
T245	Phosphorylation	Uniprot
S247	Phosphorylation	Uniprot
K311	Acetylation	Uniprot
T325	Phosphorylation	Uniprot
T327	Phosphorylation	Uniprot

Site	PTM Type	Source
S24	Phosphorylation	Uniprot
S199	Phosphorylation	Uniprot
K253	Sumoylation	Uniprot
K253	Ubiquitination	Uniprot
S267	Phosphorylation	Uniprot
T276	Phosphorylation	Uniprot
S278	Phosphorylation	Uniprot
T328	Phosphorylation	Uniprot
K342	Acetylation	Uniprot
T356	Phosphorylation	Uniprot
T358	Phosphorylation	Uniprot

Site	PTM Type	Source
S18	Phosphorylation	Uniprot
S59	Phosphorylation	Uniprot
K248	Sumoylation	Uniprot
T351	Phosphorylation	Uniprot
T353	Phosphorylation	Uniprot

研究背景

功能:

Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3' (By similarity).

细胞定位:

Nucleus. Cytoplasm.
Note: Localizes in the cytoplasm in presence of MDFIC overexpression.

组织特异性:

亚基结构:

Interacts (via the C2H2-type domains 3, 4 and 5) with MDFIC (via the C2H2-type domains 3, 4 and 5). Interacts with GLI1; the interaction enhances transcription activation. Interacts with GLI2. Interacts with GLI3; the interaction enhances transcription activation (By similarity).

蛋白家族:

The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.

Belongs to the GLI C2H2-type zinc-finger protein family.

功能:

Acts as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos.

翻译修饰:

Phosphorylated.

Ubiquitinated by RNF180, leading to its degradation.

细胞定位:

Nucleus. Cytoplasm.
Note: Localizes in the cytoplasm in presence of MDFIC overexpression. Both phosphorylated and unphosphorylated forms are localized in the nucleus (By similarity).

亚基结构:

Interacts with RNF180. Interacts (via the C2H2-type domains 3, 4 and 5) with MDFIC (via the C2H2-type domains 3, 4 and 5); the interaction reduces its transcriptional activity. Interacts with GLI1 and GLI2 (By similarity). Interacts (via C2H2-type domain 3) with DHX9.

蛋白家族:

The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.

Belongs to the GLI C2H2-type zinc-finger protein family.

功能:

Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.

细胞定位:

Nucleus. Cytoplasm.
Note: Localizes in the cytoplasm in presence of MDFIC overexpression (By similarity). Translocation to the nucleus requires KPNA1 or KPNA6.

亚基结构:

Interacts (via the C2H2-type domains 3, 4 and 5) with MDFIC (via the C2H2-type domains 3, 4 and 5); the interaction reduces its transcriptional activity (By similarity). Interacts with KPNA1 and KPNA6. Interacts (via C2H2-type domains 3, 4 and 5) with GLI3; the interaction enhances its transcriptional activity.

蛋白家族:

The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.

Belongs to the GLI C2H2-type zinc-finger protein family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

文献引用

1). The expression status of ZIC2 as a prognostic marker for nasopharyngeal carcinoma. International Journal of Clinical and Experimental Pathology, 2018 (PubMed: 31949842) [IF=1.4]

Application: IHC Species: Human Sample: NPC tissues

Figure 2 Expression analysis of ZIC2 protein as determined by immunohistochemistry (200×). ZIC2 expression was localized in the nuclei and cytoplasms of NPC cells. A. Negative staining of ZIC2 in NPC tissues. B. “+” (score 1-4, weakly positive) expression of ZIC2 in NPC tissues. C. “++” (score 5-8, positive) expression of ZIC2 in NPC tissues. D. “+++” (score 9-12, strongly positive) expression of ZIC2 in NPC tissues.

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ZIC1/2/3 Antibody - #AF0340

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MS Report

HPLC Report

MSDS

说明书

COA

实验方案

WB Handbook

IHC Protocol

IF/ICC Protocol

产品描述

抗原和靶标

种属预测

翻译修饰 - Q15915/O95409/O60481 作为底物

研究背景

研究领域

文献引用

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