产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4364, RRID:AB_2836732.
展开/折叠
mitochondrial; OTTHUMP00000235155; OTTHUMP00000235156; PC; PCB; Pcx; PYC_HUMAN; Pyruvate carboxylase; Pyruvate carboxylase mitochondrial; Pyruvic carboxylase;
抗原和靶标
- P11498 PYC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P11498 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S20 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| Y67 | Phosphorylation | Uniprot | |
| T72 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| Y118 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| Y210 | Phosphorylation | Uniprot | |
| Y217 | Phosphorylation | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| Y304 | Phosphorylation | Uniprot | |
| T309 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| T513 | Phosphorylation | Uniprot | |
| T514 | Phosphorylation | Uniprot | |
| K589 | Ubiquitination | Uniprot | |
| T824 | Phosphorylation | Uniprot | |
| S835 | Phosphorylation | Uniprot | |
| Y837 | Phosphorylation | Uniprot | |
| K892 | Ubiquitination | Uniprot | |
| Y894 | Phosphorylation | Uniprot | |
| S927 | Phosphorylation | Uniprot | |
| K969 | Ubiquitination | Uniprot | |
| K992 | Acetylation | Uniprot | |
| K992 | Ubiquitination | Uniprot | |
| T1003 | Phosphorylation | Uniprot | |
| S1009 | Phosphorylation | Uniprot | |
| K1090 | Acetylation | Uniprot | |
| T1156 | Phosphorylation | Uniprot |
研究背景
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Acetylation of Lys-748 might play a role in catalytic activity regulation.
Mitochondrion matrix.
Homotetramer. Interacts (via the biotin carboxylation domain) with SIRT4.
研究领域
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
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