PDHK1 Antibody - #DF4365

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产品: PDHK1 抗体
货号: DF4365
描述: Rabbit polyclonal antibody to PDHK1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken
分子量: 49 KD; 49kD(Calculated).
蛋白号: Q15118
RRID: AB_2836733

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Dog(82%), Chicken(80%)
克隆:
Polyclonal
特异性:
PDHK1 Antibody detects endogenous levels of total PDHK1.
RRID:
AB_2836733
引用格式: Affinity Biosciences Cat# DF4365, RRID:AB_2836733.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; HGNC:8809; Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; PDH kinase 1; Pdk1; PDK1_HUMAN; Pyruvate dehydrogenase kinase isoform 1; Pyruvate dehydrogenase kinase, isoenzyme 1;

抗原和靶标

免疫原:
Uniprot:

Q15118(PDK1_HUMAN) >>Visit HPA database.

基因/基因ID:
PDK1(5163)
表达:
Q15118 PDK1_HUMAN:

Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.

序列:
MRLARLLRGAALAGPGPGLRAAGFSRSFSSDSGSSPASERGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
91
Sheep
91
Dog
82
Chicken
80
Xenopus
78
Zebrafish
67
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15118 作为底物

Site PTM Type Enzyme
S25 Phosphorylation
S67 Phosphorylation
K133 Ubiquitination
Y136 Phosphorylation P11362 (FGFR1)
K230 Ubiquitination
Y243 Phosphorylation P11362 (FGFR1) , O60674 (JAK2) , P00519 (ABL1) , P36888 (FLT3)
Y244 Phosphorylation P11362 (FGFR1)
R319 Methylation
Y336 Phosphorylation
T338 Phosphorylation P00558 (PGK1)
T346 Phosphorylation
S347 Phosphorylation
Y357 Phosphorylation
S393 Phosphorylation
S396 Phosphorylation
Y403 Phosphorylation
K405 Ubiquitination

翻译修饰 - Q15118 作为激酶

Substrate Site Source
P05106-1 (ITGB3) T779 Uniprot
P08559 (PDHA1) S232 Uniprot
P08559 (PDHA1) S293 Uniprot
P08559 (PDHA1) S300 Uniprot
P23443-1 (RPS6KB1) T390 Uniprot
P29803 (PDHA2) S230 Uniprot
P29803 (PDHA2) S291 Uniprot
P29803 (PDHA2) S298 Uniprot
P31749-1 (AKT1) T308 Uniprot
P31749-1 (AKT1) S473 Uniprot
P31751 (AKT2) T309 Uniprot
Q15118-1 (PDK1) S25 Uniprot
Q15118-1 (PDK1) S393 Uniprot
Q15118-1 (PDK1) S396 Uniprot
Q16513 (PKN2) T816 Uniprot
Q16513-1 (PKN2) T951 Uniprot
Q9Y243-1 (AKT3) S120 Uniprot
Q9Y243 (AKT3) T305 Uniprot

研究背景

功能:

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

翻译修饰:

Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.

亚基结构:

Homodimer, and heterodimer with PDK2. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3).

蛋白家族:

Belongs to the PDK/BCKDK protein kinase family.

研究领域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

文献引用

1). Pimozide inhibits the growth of breast cancer cells by alleviating the Warburg effect through the P53 signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 35658233) [IF=7.5]

Application: WB    Species: Human    Sample: MCF-7 cells

Fig. 3. Pimozide inhibits PKM2 protein and mRNA in both MCF-7 and MDA-MB-231 cells in vitro. (A-B) Cells were treated with the indicated concentrations of Pimozide for 24 h, and the protein expression of glycolytic enzymes in MCF-7(A) and MDA-MB-231(B) cells were determined by Western blot analysis (left panel). Densitometry analysis was performed to assess the glycolytic enzymes protein expression (normalized to β-actin expression), PKM2 decreased significantly compared with untreated cells (right panel). (C-D) The mRNA expression of PKM2 in MCF-7(C) and MDA-MB-231(D) cells untreated or treated with Pimozide was determined by qRT-PCR. GAPDH was used as a control. Data represent mean ± SD from three biological replicates (*p < 0.05, **p < 0.01).
2). Lactate accumulation induced by Akt2-PDK1 signaling promotes pulmonary fibrosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2024 (PubMed: 38226859) [IF=4.8]
3). Sevoflurane preconditioning attenuates hypoxia/reoxygenation injury of H9c2 cardiomyocytes by activation of the HIF-1/PDK-1 pathway. PeerJ, 2023 (PubMed: 33391885) [IF=2.7]

Application: WB    Species: Rat    Sample: H9c2 cardiomyocytes

Figure 5 SPC upregulate the protein expression of HIF-1a and PDK-1. (A) Western blot image of HIF-1a protein. (B) Western blot image of PDK-1 protein. (C) HIF-1a protein expression. (D) PDK-1 protein expression. Protein content was normalized to GAPDH. Data represent mean ± SD (n = 3/group). *P < 0.05 vs Con, #P < 0.05 vs H/R, &P < 0.05 vs SPC.

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