产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4367, RRID:AB_2836735.
展开/折叠
ODPA_HUMAN; PDH; PDHA; PDHA1; PDHCE1A; PDHE1 A type I; PDHE1-A type I; PHE1A; Pyruvate Dehydrogenase (lipoamide) alpha 1; Pyruvate dehydrogenase complex, E1 alpha polypeptide 1; Pyruvate Dehydrogenase E1 alpha; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial;
抗原和靶标
- P08559 ODPA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRKMLAAVSRVLSGASQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P08559 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K18 | Acetylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| K39 | Acetylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K77 | Acetylation | Uniprot | |
| K83 | Acetylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| T116 | Phosphorylation | Uniprot | |
| Y118 | Phosphorylation | Uniprot | |
| T139 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| Y227 | Phosphorylation | Uniprot | |
| T231 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Q15118 (PDK1) , O15530 (PDPK1) , Q15119 (PDK2) | Uniprot |
| T240 | Phosphorylation | Uniprot | |
| Y242 | Phosphorylation | Uniprot | |
| Y243 | Phosphorylation | Uniprot | |
| K244 | Acetylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| R245 | Methylation | Uniprot | |
| Y272 | Phosphorylation | Uniprot | |
| K277 | Acetylation | Uniprot | |
| Y289 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Q16654 (PDK4) , Q15119 (PDK2) , O15530 (PDPK1) , Q15120 (PDK3) , Q15118 (PDK1) | Uniprot |
| S295 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Q15119 (PDK2) , Q15118 (PDK1) , Q16654 (PDK4) , Q15120 (PDK3) , O15530 (PDPK1) | Uniprot |
| Y301 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| K313 | Acetylation | Uniprot | |
| K321 | Acetylation | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| K336 | Acetylation | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| Y366 | Phosphorylation | Uniprot | |
| Y369 | Phosphorylation | Uniprot | |
| K385 | Methylation | Uniprot |
研究背景
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.
Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity).
Mitochondrion matrix.
Ubiquitous.
Heterotetramer of two PDHA1 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules.
研究领域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
限制条款
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