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| 产品: | RAPGEF3 抗体 |
| 货号: | DF4381 |
| 描述: | Rabbit polyclonal antibody to RAPGEF3 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 110/100 KD; 104kD(Calculated). |
| 蛋白号: | O95398 |
| RRID: | AB_2836736 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4381, RRID:AB_2836736.
展开/折叠
bcm910; CAMP GEFI; cAMP regulated guanine nucleotide exchange factor I; CAMPGEFI; CGEF 1; CGEF1; EPA1; Epac 1; EPAC; EPAC1; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; MGC21410; RAP guanine nucleotide exchange factor; Rap guanine nucleotide exchange factor (GEF) 3; RAP guanine nucleotide exchange factor 3; Rap1 guanine nucleotide exchange factor directly activated by cAMP; RAPGEF3;
抗原和靶标
Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.
- O95398 RPGF3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKVGWPGESCWQVGLAVEDSPALGAPRVGALPDVVPEGTLLNMVLRRMHRPRSCSYQLLLEHQRPSCIQGLRWTPLTNSEESLDFSESLEQASTERVLRAGRQLHRHLLATCPNLIRDRKYHLRLYRQCCSGRELVDGILALGLGVHSRSQVVGICQVLLDEGALCHVKHDWAFQDRDAQFYRFPGPEPEPVRTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMGPDSSAHDPTETFLSDFLLTHRVFMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALAKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95398 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S82 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| K268 | Acetylation | Uniprot | |
| T380 | Phosphorylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S864 | Phosphorylation | Uniprot |
研究背景
Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.
Endomembrane system.
Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.
Interacts with PDE3B.
The DEP domain is involved in membrane localization independent from regulation by cAMP.
研究领域
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Serotonergic synapse.
文献引用
Application: WB Species: rat Sample: HEI-OC1 cells
Application: IF/ICC Species: rat Sample: HEI-OC1 cells
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