RAPGEF3 Antibody - #DF4381

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产品: RAPGEF3 抗体
货号: DF4381
描述: Rabbit polyclonal antibody to RAPGEF3
应用: WB IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: O95398
RRID: AB_2836736

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
RAPGEF3 Antibody detects endogenous levels of total RAPGEF3.
RRID:
AB_2836736
引用格式: Affinity Biosciences Cat# DF4381, RRID:AB_2836736.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bcm910; CAMP GEFI; cAMP regulated guanine nucleotide exchange factor I; CAMPGEFI; CGEF 1; CGEF1; EPA1; Epac 1; EPAC; EPAC1; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; MGC21410; RAP guanine nucleotide exchange factor; Rap guanine nucleotide exchange factor (GEF) 3; RAP guanine nucleotide exchange factor 3; Rap1 guanine nucleotide exchange factor directly activated by cAMP; RAPGEF3;

抗原和靶标

免疫原:

A synthesized peptide derived from human RAPGEF3, corresponding to a region within C-terminal amino acids.

基因/基因ID:
RAPGEF3(10411)

研究领域

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Serotonergic synapse.

文献引用

1). Epac1 signaling pathway mediates the damage and apoptosis of inner ear hair cells after noise exposure in a rat model. NEUROSCIENCE, 2021 (PubMed: 33838290) [IF=2.9]

Application: WB    Species: rat    Sample: HEI-OC1 cells

Fig. 6. Expression of Epac1 in HEI-OC1 cells after oligomycin exposure. (A) Representative fluorescent microscopic images showing Epac1 expression in HEI-OC1 cells 12 h after oligomycin exposure; blue: DAPI; scale bar = 20 mm, n = 3. (B) Bar graph representing the intensity of Epac1 signal in HEI-OC1 cells. (C) Western blot analysis of Epac1 and Rap1 expression in HEI-OC1 cells 12 h after oligomycin exposure. b-actin was used as the loading control. The data represent the mean ± SEM, n = 3; *P < 0.05 vs. Control group, #P < 0.05 vs. OA.

Application: IF/ICC    Species: rat    Sample: HEI-OC1 cells

Fig. 6. Expression of Epac1 in HEI-OC1 cells after oligomycin exposure. (A) Representative fluorescent microscopic images showing Epac1 expression in HEI-OC1 cells 12 h after oligomycin exposure; blue: DAPI; scale bar = 20 mm, n = 3. (B) Bar graph representing the intensity of Epac1 signal in HEI-OC1 cells. (C) Western blot analysis of Epac1 and Rap1 expression in HEI-OC1 cells 12 h after oligomycin exposure. b-actin was used as the loading control. The data represent the mean ± SEM, n = 3; *P < 0.05 vs. Control group, #P < 0.05 vs. OA.

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