(1)
(6)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4383, RRID:AB_2836738.
展开/折叠
KIAA0474; Rap1 GTPase activating protein 1; RAP1 GTPase activating protein; Rap1 GTPase-activating protein 1; RAP1GA1; Rap1ga1 protein; Rap1GAP; Rap1GAP1; RAP1GAPII; RAPGAP; RPGP1_HUMAN;
抗原和靶标
Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.
- P47736 RPGP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIEKMQGSRMDEQRCSFPPPLKTEEDYIPYPSVHEVLGREGPFPLILLPQFGGYWIEGTNHEITSIPETEPLQSPTTKVKLECNPTARIYRKHFLGKEHFNYYSLDAALGHLVFSLKYDVIGDQEHLRLLLRTKCRTYHDVIPISCLTEFPNVVQMAKLVCEDVNVDRFYPVLYPKASRLIVTFDEHVISNNFKFGVIYQKLGQTSEEELFSTNEESPAFVEFLEFLGQKVKLQDFKGFRGGLDVTHGQTGTESVYCNFRNKEIMFHVSTKLPYTEGDAQQLQRKRHIGNDIVAVVFQDENTPFVPDMIASNFLHAYVVVQAEGGGPDGPLYKVSVTARDDVPFFGPPLPDPAVFRKGPEFQEFLLTKLINAEYACYKAEKFAKLEERTRAALLETLYEELHIHSQSMMGLGGDEDKMENGSGGGGFFESFKRVIRSRSQSMDAMGLSNKKPNTVSTSHSGSFAPNNPDLAKAAGISLIVPGKSPTRKKSGPFGSRRSSAIGIENIQEVQEKRESPPAGQKTPDSGHVSQEPKSENSSTQSSPEMPTTKNRAETAAQRAEALKDFSRSSSSASSFASVVEETEGVDGEDTGLESVSSSGTPHKRDSFIYSTWLEDSVSTTSGGSSPGPSRSPHPDAGKLGDPACPEIKIQLEASEQHMPQLGC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P47736 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y27 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K262 | Acetylation | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| K378 | Acetylation | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K384 | Acetylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T486 | Phosphorylation | Uniprot | |
| S490 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S495 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S515 | Phosphorylation | Uniprot | |
| T522 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S541 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| T547 | Phosphorylation | Uniprot | |
| T548 | Phosphorylation | Uniprot | |
| S625 | Phosphorylation | Uniprot |
研究背景
GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
Golgi apparatus membrane>Peripheral membrane protein.
Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.
Homodimer and heterodimer with RAP1B.
研究领域
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
文献引用
Application: WB Species: Mouse Sample: skin tissues
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