产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4394, RRID:AB_2836749.
展开/折叠
AU041672; FLJ13311; GTPase interacting protein 2; GTPase-interacting protein 2; GTR2; MGC47404; OTTHUMP00000000548; Rag C; Rag C protein; RAGC; Ras related GTP binding C; Ras-related GTP-binding protein C; RP23 29H22.4; RRAGC; RRAGC_HUMAN; TIB929; YGR163W;
抗原和靶标
- Q9HB90 RRAGC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLQYGAEETPLAGSYGAADSFPKDFGYGVEEEEEEAAAAGGGVGAGAGGGCGPGGADSSKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKIYKDDISNSSFVNFQIWDFPGQMDFFDPTFDYEMIFRGTGALIYVIDAQDDYMEALTRLHITVSKAYKVNPDMNFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKLHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSSPVDMQSYELCCDMIDVVIDVSCIYGLKEDGSGSAYDKESMAIIKLNNTTVLYLKEVTKFLALVCILREESFERKGLIDYNFHCFRKAIHEVFEVGVTSHRSCGHQTSASSLKALTHNGTPRNAI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9HB90 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| Y16 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| Y327 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| T381 | Phosphorylation | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| K387 | Methylation | Uniprot | |
| T394 | Phosphorylation | Uniprot |
研究背景
Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
Cytoplasm. Nucleus. Lysosome.
Note: Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA (PubMed:11073942).
Forms a heterodimer with RRAGA, in a sequence-independent manner, and RRAGB. Heterodimerization stabilizes proteins of the heterodimer. In complex with RRAGA or RRAGB, interacts with RPTOR; this interaction is particularly efficient with GTP-loaded RRAGB and GDP-loaded RRAGC. Interacts with NOL8. Interacts with SH3BP4; the interaction with this negative regulator is most probably direct, preferentially occurs with the inactive GDP-bound form of RRAGB, is negatively regulated by amino acids and prevents interaction with RPTOR. The Rag heterodimer interacts with SLC38A9; the probable amino acid sensor. Interacts with SESN1, SESN2 AND SESN3. Interacts with PIP4P1 (By similarity).
Belongs to the GTR/RAG GTP-binding protein family.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
限制条款
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