ALDH1A2 Antibody - #DF4422

Figure 1. Transferrin is a potential contributory factor for intestinal tolerance dependent on gut microbiota. (A) Transferrin concentration in plasma of germ-free (GF) and specific pathogen-free (SPF) (control) mice determined by ELISA. Data represent means ± SD (n = 10), *p < 0.05, **p < 0.01 by one-way ANOVA with Dunnett’s post-hoc test. Western blotting (left) and quantification (right) analyses of transferrin levels in protein extracts from liver (B) and spleen (C) of SPF and GF mice. β-actin was used as the control. Data represent means ± SD (n = 10), *p < 0.05, **p < 0.01 by one-way ANOVA with Dunnett’s post-hoc test (B, C). (D-E) Transferrin, ALDH1A2, CCL22, TGF-β, and IL-10 levels in different gut tissue segments (duodenum, jejunum, ileum, caecum, and colon (D, J, I, C1, and C2)) of SPF (NC), GF (D), and broad-spectrum antibiotic (Abs)-treated SPF (E) mice were determined by Western blotting (left) and quantification (right) analyses. GAPDH was used as the control. Data represent means ± SD (n = 8),