产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4426, RRID:AB_2836781.
展开/折叠
MST066; MST110; MSTP038; MSTP066; MSTP110; NADRIN; Neuron associated developmentally regulated protein; PP367; PP4534; Rho GTPase activating protein 17; Rho type GTPase activating protein 17; RhoGAP interacting with CIP4 homologs 1; RhoGAP interacting with CIP4 homologs protein 1; RICH 1; RICH1; RICH1B; WBP15;
抗原和靶标
- Q68EM7 RHG17_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKKQFNRMKQLANQTVGRAEKTEVLSEDLLQIERRLDTVRSICHHSHKRLVACFQGQHGTDAERRHKKLPLTALAQNMQEASTQLEDSLLGKMLETCGDAENQLALELSQHEVFVEKEIVDPLYGIAEVEIPNIQKQRKQLARLVLDWDSVRARWNQAHKSSGTNFQGLPSKIDTLKEEMDEAGNKVEQCKDQLAADMYNFMAKEGEYGKFFVTLLEAQADYHRKALAVLEKTLPEMRAHQDKWAEKPAFGTPLEEHLKRSGREIALPIEACVMLLLETGMKEEGLFRIGAGASKLKKLKAALDCSTSHLDEFYSDPHAVAGALKSYLRELPEPLMTFNLYEEWTQVASVQDQDKKLQDLWRTCQKLPPQNFVNFRYLIKFLAKLAQTSDVNKMTPSNIAIVLGPNLLWARNEGTLAEMAAATSVHVVAVIEPIIQHADWFFPEEVEFNVSEAFVPLTTPSSNHSFHTGNDSDSGTLERKRPASMAVMEGDLVKKESFGVKLMDFQAHRRGGTLNRKHISPAFQPPLPPTDGSTVVPAGPEPPPQSSRAESSSGGGTVPSSAGILEQGPSPGDGSPPKPKDPVSAAVPAPGRNNSQIASGQNQPQAAAGSHQLSMGQPHNAAGPSPHTLRRAVKKPAPAPPKPGNPPPGHPGGQSSSGTSQHPPSLSPKPPTRSPSPPTQHTGQPPGQPSAPSQLSAPRRYSSSLSPIQAPNHPPPQPPTQATPLMHTKPNSQGPPNPMALPSEHGLEQPSHTPPQTPTPPSTPPLGKQNPSLPAPQTLAGGNPETAQPHAGTLPRPRPVPKPRNRPSVPPPPQPPGVHSAGDSSLTNTAPTASKIVTDSNSRVSEPHRSIFPEMHSDSASKDVPGRILLDIDNDTESTAL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q68EM7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K3 | Methylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K48 | Acetylation | Uniprot | |
| T72 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| T83 | Phosphorylation | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| K295 | Acetylation | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| Y314 | Phosphorylation | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| S625 | Phosphorylation | Uniprot | |
| T628 | Phosphorylation | Uniprot | |
| S667 | Phosphorylation | Uniprot | |
| T672 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| S676 | Phosphorylation | Uniprot | |
| T679 | Phosphorylation | Uniprot | |
| T682 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| S702 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S703 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S706 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| T753 | Phosphorylation | Uniprot | |
| T757 | Phosphorylation | Uniprot | |
| T759 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| T763 | Phosphorylation | Uniprot | |
| T793 | Phosphorylation | Uniprot | |
| S808 | Phosphorylation | Uniprot | |
| S857 | Phosphorylation | Uniprot |
研究背景
Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1.
Membrane>Peripheral membrane protein. Cytoplasm. Cell junction>Tight junction.
Note: Associates with membranes and concentrates at sites of cell-cell contact.
Ubiquitously expressed. Expressed at higher level in heart and placenta.
Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, PATJ and PARD3/PAR3. Interacts with SLC9A3R1, FNBP1, TRIP10, CAPZA (CAPZA1, CAPZA2 or CAPZA3), CAPZB, CD2AP and SH3KBP1/CIN85.
The BAR domain mediates the interaction with the coiled coil domain of AMOT, leading to its recruitment to tight junctions.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.