产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4428, RRID:AB_2836783.
展开/折叠
10C; ARHGAP9; FLJ16525; MGC1295; RGL1; RHG09_HUMAN; Rho GTPase activating protein 9; Rho GTPase-activating protein 9; Rho type GTPase activating protein 9; Rho-type GTPase-activating protein 9;
抗原和靶标
- Q9BRR9 RHG09_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLSSRWWPSSWGILGLGPRSPPRGSQLCALYAFTYTGADGQQVSLAEGDRFLLLRKTNSDWWLARRLEAPSTSRPIFVPAAYMIEESIPSQSPTTVIPGQLLWTPGPKLFHGSLEELSQALPSRAQASSEQPPPLPRKMCRSVSTDNLSPSLLKPFQEGPSGRSLSQEDLPSEASASTAGPQPLMSEPPVYCNLVDLRRCPRSPPPGPACPLLQRLDAWEQHLDPNSGRCFYINSLTGCKSWKPPRRSRSETNPGSMEGTQTLKRNNDVLQPQAKGFRSDTGTPEPLDPQGSLSLSQRTSQLDPPALQAPRPLPQLLDDPHEVEKSGLLNMTKIAQGGRKLRKNWGPSWVVLTGNSLVFYREPPPTAPSSGWGPAGSRPESSVDLRGAALAHGRHLSSRRNVLHIRTIPGHEFLLQSDHETELRAWHRALRTVIERLVRWVEARREAPTGRDQGSGDRENPLELRLSGSGPAELSAGEDEEEESELVSKPLLRLSSRRSSIRGPEGTEQNRVRNKLKRLIAKRPPLQSLQERGLLRDQVFGCQLESLCQREGDTVPSFLRLCIAAVDKRGLDVDGIYRVSGNLAVVQKLRFLVDRERAVTSDGRYVFPEQPGQEGRLDLDSTEWDDIHVVTGALKLFLRELPQPLVPPLLLPHFRAALALSESEQCLSQIQELIGSMPKPNHDTLRYLLEHLCRVIAHSDKNRMTPHNLGIVFGPTLFRPEQETSDPAAHALYPGQLVQLMLTNFTSLFP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BRR9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S20 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| Y191 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| Y232 | Phosphorylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| T283 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| R386 | Methylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| K489 | Ubiquitination | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S496 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| S500 | Phosphorylation | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| K588 | Ubiquitination | Uniprot |
研究背景
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has a substantial GAP activity toward CDC42 and RAC1 and less toward RHOA. Has a role in regulating adhesion of hematopoietic cells to the extracellular matrix. Binds phosphoinositides, and has the highest affinity for phosphatidylinositol 3,4,5-trisphosphate, followed by phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate.
Predominantly expressed in peripheral blood leukocytes, spleen, and thymus.
Interacts with FASLG.
A region including the PH domain and partially overlapping with the Rho-GAP domain mediates interaction with phosphoinositides.
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