| 产品: | ARHGEF1 抗体 |
| 货号: | DF4429 |
| 描述: | Rabbit polyclonal antibody to ARHGEF1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Sheep, Dog |
| 分子量: | 105 KD; 102kD(Calculated). |
| 蛋白号: | Q92888 |
| RRID: | AB_2836784 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4429, RRID:AB_2836784.
展开/折叠
115 kD protein; 115 kDa guanine nucleotide exchange factor; ARHG1_HUMAN; ARHGEF 1; ARHGEF1; GEF 1; GEF1; LBCL 2; LBCL2; LSC; Lsc homolog; p115 RhoGEF; p115-RhoGEF; p115RhoGEF; Rho guanine nucleotide exchange factor (GEF) 1; Rho guanine nucleotide exchange factor 1; Sub1.5;
抗原和靶标
- Q92888 ARHG1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLGSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSGLELEPEEPPGWRELVPPDTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLEELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADARTERILSDLLPFCRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92888 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K234 | Acetylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| Y487 | Phosphorylation | Uniprot | |
| K510 | Ubiquitination | Uniprot | |
| K527 | Ubiquitination | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| S625 | Phosphorylation | Uniprot | |
| S630 | Phosphorylation | Uniprot | |
| S631 | Phosphorylation | Uniprot | |
| K639 | Ubiquitination | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| K688 | Ubiquitination | Uniprot | |
| T695 | Phosphorylation | Uniprot | |
| K701 | Ubiquitination | Uniprot | |
| Y738 | Phosphorylation | O60674 (JAK2) | Uniprot |
| K763 | Ubiquitination | Uniprot | |
| S776 | Phosphorylation | Uniprot | |
| S786 | Phosphorylation | Uniprot | |
| S788 | Phosphorylation | Uniprot | |
| S798 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| S833 | Phosphorylation | Uniprot | |
| K835 | Ubiquitination | Uniprot | |
| S863 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| T912 | Phosphorylation | Uniprot |
研究背景
Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.
Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2.
Cytoplasm. Membrane.
Note: Translocated to the membrane by activated GNA13 or LPA stimulation.
Ubiquitously expressed.
Interacts with RHOA, GNA12 and GNA13. Homooligomerizes through the coiled coil region. May interact with CCPG1 (By similarity). Interacts with CTNNAL1.
The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.
The DH domain is involved in interaction with CCPG1.
研究领域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
限制条款
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