产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4431, RRID:AB_2836786.
展开/折叠
ARHGB_HUMAN; ARHGEF 11; ARHGEF11; DKFZp667F1223; Glutamate transporter EAAT4 associated protein 48; GTRAP 48; GTRAP48; KIAA0380; OTTHUMP00000038716; OTTHUMP00000038717; PDZ RHOGEF; PDZ-RhoGEF; Rho guanine exchange factor (GEF) 11; Rho guanine nucleotide exchange factor (GEF) 11; Rho guanine nucleotide exchange factor 11; RhoA specific guanine nucleotide exchange factor; RhoGEF glutamate transport modulator;
抗原和靶标
- O15085 ARHGB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVRLPQSIDRLSSLSSLGDSAPERKSPSHHRQPSDASETTGLVQRCVIIQKDQHGFGFTVSGDRIVLVQSVRPGGAAMKAGVKEGDRIIKVNGTMVTNSSHLEVVKLIKSGAYVALTLLGSSPSSMGISGLQQDPSPAGAPRITSVIPSPPPPPPLPPPQRITGPKPLQDPEVQKHATQILRNMLRQEEKELQDILPLYGDTSQRPSEGRLSLDSQEGDSGLDSGTERFPSLSESLMNRNSVLSDPGLDSPRTSPVIMARVAQHHRRQGSDAAVPSTGDQGVDQSPKPLIIGPEEDYDPGYFNNESDIIFQDLEKLKSRPAHLGVFLRYIFSQADPSPLLFYLCAEVYQQASPKDSRSLGKDIWNIFLEKNAPLRVKIPEMLQAEIDSRLRNSEDARGVLCEAQEAAMPEIQEQIHDYRTKRTLGLGSLYGENDLLDLDGDPLRERQVAEKQLAALGDILSKYEEDRSAPMDFALNTYMSHAGIRLREARPSNTAEKAQSAPDKDKWLPFFPKTKKSSNSKKEKDALEDKKRNPILKYIGKPKSSSQSTFHIPLSPVEVKPGNVRNIIQHFENNQQYDAPEPGTQRLSTGSFPEDLLESDSSRSEIRLGRSESLKGREEMKRSRKAENVPRSRSDVDMDAAAEATRLHQSASSSTSSLSTRSLENPTPPFTPKMGRRSIESPSLGFCTDTLLPHLLEDDLGQLSDLEPEPDAQNWQHTVGKDVVAGLTQREIDRQEVINELFVTEASHLRTLRVLDLIFYQRMKKENLMPREELARLFPNLPELIEIHNSWCEAMKKLREEGPIIKEISDLMLARFDGPAREELQQVAAQFCSYQSIALELIKTKQRKESRFQLFMQEAESHPQCRRLQLRDLIISEMQRLTKYPLLLESIIKHTEGGTSEHEKLCRARDQCREILKYVNEAVKQTENRHRLEGYQKRLDATALERASNPLAAEFKSLDLTTRKMIHEGPLTWRISKDKTLDLHVLLLEDLLVLLQKQDEKLLLKCHSKTAVGSSDSKQTFSPVLKLNAVLIRSVATDKRAFFIICTSKLGPPQIYELVALTSSDKNTWMELLEEAVRNATRHPGAAPMPVHPPPPGPREPAQQGPTPSRVELDDSDVFHGEPEPEELPGGTGSQQRVQGKHQVLLEDPEQEGSAEEEELGVLPCPSTSLDGENRGIRTRNPIHLAFPGPLFMEGLADSALEDVENLRHLILWSLLPGHTMETQAAQEPEDDLTPTPSVISVTSHPWDPGSPGQAPPGGEGDNTQLAGLEGERPEQEDMGLCSLEHLPPRTRNSGIWESPELDRNLAEDASSTEAAGGYKVVRKAEVAGSKVVPALPESGQSEPGPPEVEGGTKATGNCFYVSMPSGPPDSSTDHSEAPMSPPQPDSLPAGQTEPQPQLQGGNDDPRRPSRSPPSLALRDVGMIFHTIEQLTLKLNRLKDMELAHRELLKSLGGESSGGTTPVGSFHTEAARWTDGSLSPPAKEPLASDSRNSHELGPCPEDGSDAPLEDSTADAAASPGP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15085 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| T40 | Phosphorylation | Uniprot | |
| T41 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| T421 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| T590 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| S635 | Phosphorylation | Uniprot | |
| S651 | Phosphorylation | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| S654 | Phosphorylation | Uniprot | |
| S655 | Phosphorylation | Uniprot | |
| T656 | Phosphorylation | Uniprot | |
| S657 | Phosphorylation | Uniprot | |
| S658 | Phosphorylation | Uniprot | |
| S663 | Phosphorylation | Uniprot | |
| T668 | Phosphorylation | Uniprot | |
| T672 | Phosphorylation | Uniprot | |
| S679 | Phosphorylation | Uniprot | |
| S851 | Phosphorylation | Uniprot | |
| K925 | Acetylation | Uniprot | |
| K938 | Ubiquitination | Uniprot | |
| T1063 | Phosphorylation | Uniprot | |
| S1064 | Phosphorylation | Uniprot | |
| S1065 | Phosphorylation | Uniprot | |
| S1135 | Phosphorylation | Uniprot | |
| S1155 | Phosphorylation | Uniprot | |
| S1168 | Phosphorylation | Uniprot | |
| T1292 | Phosphorylation | Uniprot | |
| S1295 | Phosphorylation | Uniprot | |
| S1300 | Phosphorylation | Uniprot | |
| S1411 | Phosphorylation | Uniprot | |
| S1413 | Phosphorylation | Uniprot | |
| S1452 | Phosphorylation | Uniprot | |
| S1457 | Phosphorylation | Uniprot | |
| S1458 | Phosphorylation | Uniprot | |
| T1461 | Phosphorylation | Uniprot | |
| T1462 | Phosphorylation | Uniprot | |
| S1466 | Phosphorylation | Uniprot | |
| T1475 | Phosphorylation | Uniprot | |
| S1478 | Phosphorylation | Uniprot | |
| S1480 | Phosphorylation | Uniprot | |
| S1489 | Phosphorylation | Uniprot | |
| S1491 | Phosphorylation | Uniprot | |
| C1500 | S-Nitrosylation | Uniprot | |
| S1519 | Phosphorylation | Uniprot |
研究背景
May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Involved in neurotrophin-induced neurite outgrowth.
Phosphorylated by MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14).
Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex when previously phosphorylated by MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14), leading to its degradation, thereby restricting RhoA activity and facilitating growth cone spreading and neurite outgrowth.
Cytoplasm. Membrane.
Note: Translocated to the membrane upon stimulation.
Ubiquitously expressed.
Interacts with GNA12 and GNA13 through the RGS domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts with SLC1A6 (By similarity). Interacts (via DH domain) with GCSAM (via C-terminus).
The poly-Pro region is essential for plasma membrane localization upon stimulation.
研究领域
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
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