产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4436, RRID:AB_2836791.
展开/折叠
ARHG7; ARHG7_HUMAN; ARHGEF 7; Arhgef7; Beta pix; Beta-Pix; betaPix; betaPix-b; betaPix-c; Cool; COOL-1; COOL1; DKFZp686C12170; DKFZp761K1021; KIAA0142; KIAA0412; mKIAA0142; Nbla10314; P50; P50BP; p85; P85COOL1; P85SPR; PAK interacting exchange factor beta; PAK-interacting exchange factor beta; PAK3; Pak3bp; PIX; PIXB; Rho guanine nucleotide exchange factor (GEF) 7; Rho guanine nucleotide exchange factor (GEF) 7b; Rho guanine nucleotide exchange factor (GEF7); Rho guanine nucleotide exchange factor 7; SH3 domain containing proline rich protein;
抗原和靶标
- Q14155 ARHG7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNSAEQTVTWLITLGVLESPKKTISDPEGFLQASLKDGVVLCRLLERLLPGTIEKVYPEPRSESECLSNIREFLRGCGASLRLELLFPPSQPPQHLVTTILLSASTFDANDLYQGQNFNKVLSSLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQSLHTRTSKLFQGQYRSLDMTDNSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTGWFPSNYVREVKASEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTYLRPLQTSEKLSSANISYLMGNLEEICSFQQMLVQSLEECTKLPEAQQRVGGCFLNLMPQMKTLYLTYCANHPSAVNVLTEHSEELGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHTDRQDIQKSMAAFKNLSAQCQEVRKRKELELQILTEAIRNWEGDDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLQEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLPSHPVTPSSKHADSKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPKTPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLADDDQPSLDSLGRRSSLSRLEPSDLSEDSDYDSIWTAHSYRMGSTSRKSCCSYISHQN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14155 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S25 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| T127 | Phosphorylation | Q5S007 (LRRK2) | Uniprot |
| S139 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| T164 | Phosphorylation | Q5S007 (LRRK2) | Uniprot |
| S176 | Phosphorylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| T264 | Phosphorylation | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| Y427 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| S518 | Phosphorylation | P05129 (PRKCG) , Q13153 (PAK1) | Uniprot |
| T528 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| K575 | Ubiquitination | Uniprot | |
| T580 | Phosphorylation | Uniprot | |
| S581 | Phosphorylation | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| T603 | Phosphorylation | Uniprot | |
| T617 | Phosphorylation | Uniprot | |
| Y620 | Phosphorylation | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| K675 | Acetylation | Uniprot | |
| S676 | Phosphorylation | Uniprot | |
| K678 | Acetylation | Uniprot | |
| K681 | Acetylation | Uniprot | |
| K682 | Acetylation | Uniprot | |
| S694 | Phosphorylation | Q14012 (CAMK1) | Uniprot |
| S700 | Phosphorylation | Uniprot | |
| K702 | Ubiquitination | Uniprot | |
| S703 | Phosphorylation | Uniprot | |
| T704 | Phosphorylation | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| Y720 | Phosphorylation | Uniprot | |
| T722 | Phosphorylation | Uniprot | |
| S723 | Phosphorylation | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| S761 | Phosphorylation | P05129 (PRKCG) | Uniprot |
| S763 | Phosphorylation | Uniprot | |
| S784 | Phosphorylation | Uniprot | |
| Y785 | Phosphorylation | Uniprot | |
| S800 | Phosphorylation | Uniprot |
研究背景
Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.
Phosphorylated by PTK2/FAK1; this promotes interaction with RAC1 (By similarity). Phosphorylated on Ser-694 by CaMK1; enhancement of GEF activity and downstream activation of RAC1.
Cell junction>Focal adhesion. Cell projection>Ruffle. Cytoplasm>Cell cortex. Cell projection>Lamellipodium.
Note: Detected at cell adhesions. A small proportion is detected at focal adhesions.
Interacts with PAK kinases through the SH3 domain. Interacts with GIT1 and TGFB1I1. Interacts with PTK2/FAK1 and RAC1. Interacts with ITCH and PARVB (By similarity). Interacts with unphosphorylated PAK1. Interacts with SCRIB; interaction is direct and may play a role in regulation of apoptosis. Interacts with FRMPD4 (via N-terminus). Interacts with CaMK1. Interacts with BIN2. Isoform 1 and isoform 5 interact with SNX27. Interacts with YWHAZ (By similarity).
研究领域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
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