| 产品: | EWSR1 抗体 |
| 货号: | DF4447 |
| 描述: | Rabbit polyclonal antibody to EWSR1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Dog, Chicken |
| 分子量: | 68 KD; 68kD(Calculated). |
| 蛋白号: | Q01844 |
| RRID: | AB_2836802 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4447, RRID:AB_2836802.
展开/折叠
bK984G1.4; bK984G1.4 Ewing sarcoma breakpoint region 1 protein; Ewing sarcoma breakpoint region 1; Ewing sarcoma breakpoint region 1 protein; Ewings sarcoma EWS Fli1 type 1 oncogene; EWS; EWS oncogene; EWS RNA binding protein 1; EWS_HUMAN; EWSR 1; Ewsr1; EWSR1 protein; RNA binding protein EWS; RNA-binding protein EWS;
抗原和靶标
- Q01844 EWS_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLADFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGLPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q01844 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T79 | Phosphorylation | P45983 (MAPK8) , P28482 (MAPK1) , Q13535 (ATR) , Q13315 (ATM) , Q16539 (MAPK14) , Q15759 (MAPK11) , P27361 (MAPK3) | Uniprot |
| S266 | Phosphorylation | P05771 (PRKCB) , P17252 (PRKCA) | Uniprot |
| Y278 | Phosphorylation | P12931 (SRC) | Uniprot |
| S286 | Phosphorylation | Uniprot | |
| R292 | Methylation | Uniprot | |
| R300 | Methylation | Uniprot | |
| R302 | Methylation | Uniprot | |
| R304 | Methylation | Uniprot | |
| R309 | Methylation | Uniprot | |
| R314 | Methylation | Uniprot | |
| R317 | Methylation | Uniprot | |
| R321 | Methylation | Uniprot | |
| R330 | Methylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| Y417 | Phosphorylation | Uniprot | |
| T422 | Phosphorylation | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| K433 | Acetylation | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K439 | Acetylation | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| R455 | Methylation | Uniprot | |
| R461 | Methylation | Uniprot | |
| R464 | Methylation | Uniprot | |
| R471 | Methylation | Uniprot | |
| R486 | Methylation | Uniprot | |
| R490 | Methylation | Uniprot | |
| R494 | Methylation | Uniprot | |
| R500 | Methylation | Uniprot | |
| R503 | Methylation | Uniprot | |
| R506 | Methylation | Uniprot | |
| R563 | Methylation | Uniprot | |
| R565 | Methylation | Uniprot | |
| R572 | Methylation | Uniprot | |
| R575 | Methylation | Uniprot | |
| R581 | Methylation | Uniprot | |
| R589 | Methylation | Uniprot | |
| R592 | Methylation | Uniprot | |
| R596 | Methylation | Uniprot | |
| R600 | Methylation | Uniprot | |
| R603 | Methylation | Uniprot | |
| R607 | Methylation | Uniprot | |
| R614 | Methylation | Uniprot | |
| R615 | Methylation | Uniprot | |
| R632 | Methylation | Uniprot | |
| R633 | Methylation | Uniprot | |
| R636 | Methylation | Uniprot | |
| Y656 | Phosphorylation | Uniprot |
研究背景
Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
Phosphorylated; calmodulin-binding inhibits phosphorylation of Ser-266.
Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8.
Nucleus. Cytoplasm. Cell membrane.
Note: Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.
Ubiquitous.
Binds POLR2C, SF1, calmodulin and RNA. Interacts with PTK2B/FAK2 and TDRD3. Binds calmodulin in the presence, but not in the absence, of calcium ion. Forms a complex with REC8, PRDM9, SYCP3 and SYCP1; complex formation is dependent of phosphorylated form of REC8 and requires PRDM9 bound to hotspot DNA; EWSR1 joins PRDM9 with the chromosomal axis through REC8 (By similarity).
EWS activation domain (EAD) functions as a potent activation domain in EFPS. EWSR1 binds POLR2C but not POLR2E or POLR2G, whereas the isolated EAD binds POLR2E and POLR2G but not POLR2C. Cis-linked RNA-binding domain (RBD) can strongly and specifically repress trans-activation by the EAD.
Belongs to the RRM TET family.
研究领域
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
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