产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4459, RRID:AB_2836814.
展开/折叠
KIAA0128; Nkrf; RCG53214, isoform CRA_d; RP5-876A24.2; SEP2; SEPT2; SEPT6; SEPT6/MLL FUSION GENE; SEPT6_HUMAN; Septin 2; Septin 6; Septin-6;
抗原和靶标
- Q14141 SEPT6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAATDIARQVGEGCRTVPLAGHVGFDSLPDQLVNKSVSQGFCFNILCVGETGLGKSTLMDTLFNTKFEGEPATHTQPGVQLQSNTYDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVLHTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADAISKSELTKFKIKITSELVSNGVQIYQFPTDDESVAEINGTMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEDKKKSLDDEVNAFKQRKTAAELLQSQGSQAGGSQTLKRDKEKKNNPWLCTE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14141 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| Y295 | Phosphorylation | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| Y324 | Phosphorylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| K362 | Acetylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K367 | Acetylation | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| K379 | Acetylation | Uniprot | |
| K380 | Acetylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| K397 | Acetylation | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| T418 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| C432 | S-Nitrosylation | Uniprot |
研究背景
Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation.
Cytoplasm. Cytoplasm>Cytoskeleton>Spindle. Chromosome>Centromere>Kinetochore. Cleavage furrow. Midbody. Cell projection>Cilium>Flagellum.
Note: In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. Found in the sperm annulus (PubMed:25588830).
Widely expressed.
Septins polymerize into heterooligomeric protein complexes that form filaments, and associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation. Filaments are assembled from asymmetrical heterotrimers, composed of SEPTIN2, SEPTIN6 and SEPTIN7 that associate head-to-head to form a hexameric unit. Within the trimer, directly interacts with SEPTIN2 and SEPTIN7. Also interacts with SEPTIN9 and SEPTIN12. Interaction with SEPTIN12 alters filament structure. Component of a septin core octomeric complex consisting of SEPTIN12, SEPTIN7, SEPTIN6 and SEPTIN2 or SEPTIN4 in the order 12-7-6-2-2-6-7-12 or 12-7-6-4-4-6-7-12 and located in the sperm annulus. Interacts with SOCS7. Interacts with HNRNPA1.
(Microbial infection) Interacts with HCV NS5B.
Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.
研究领域
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
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