NEK7 Antibody - #DF4467

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产品: NEK7 抗体
货号: DF4467
描述: Rabbit polyclonal antibody to NEK7
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 35 KD; 35kD(Calculated).
蛋白号: Q8TDX7
RRID: AB_2836822

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
NEK7 Antibody detects endogenous levels of total NEK7.
RRID:
AB_2836822
引用格式: Affinity Biosciences Cat# DF4467, RRID:AB_2836822.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 2.7.11.1; NEK7; NEK7_HUMAN; Never in mitosis A-related kinase 7; Never in mitosis gene a-related kinase 7; NIMA (never in mitosis gene a) related kinase 7; NIMA (never in mitosis gene a)-related expressed kinase 7; NimA related protein kinase 7; NIMA-related kinase 7; NimA-related protein kinase 7; Serine/threonine-protein kinase Nek7;

抗原和靶标

免疫原:
Uniprot:

Q8TDX7(NEK7_HUMAN) >>Visit HPA database.

基因/基因ID:
NEK7(140609)
表达:
Q8TDX7 NEK7_HUMAN:

Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.

序列:
MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
78
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8TDX7 作为底物

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
K20 Ubiquitination
T30 Phosphorylation
K63 Ubiquitination
K64 Ubiquitination
K74 Ubiquitination
K81 Ubiquitination
K87 Ubiquitination
Y97 Phosphorylation
S147 Phosphorylation
K163 Ubiquitination
T170 Phosphorylation
T172 Phosphorylation
K176 Ubiquitination
S187 Phosphorylation
S188 Phosphorylation
K189 Ubiquitination
T190 Phosphorylation
T191 Phosphorylation
S195 Phosphorylation Q8TD19 (NEK9)
T199 Phosphorylation
Y201 Phosphorylation
Y202 Phosphorylation
S204 Phosphorylation
Y244 Phosphorylation
S245 Phosphorylation
K248 Ubiquitination
K249 Ubiquitination
K281 Ubiquitination
T286 Phosphorylation
Y287 Phosphorylation
Y289 Phosphorylation
K293 Ubiquitination

翻译修饰 - Q8TDX7 作为激酶

Substrate Site Source
P23443 (RPS6KB1) T412 Uniprot
P54274 (TERF1) S114 Uniprot

研究背景

功能:

Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.

翻译修饰:

Phosphorylation at Ser-195 required for its activation.

细胞定位:

Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.

亚基结构:

Monomer. Interacts with NEK9. Interacts with ANKS3; this interaction alters the subcellular distribution of NEK7 by preventing its nuclear translocation.

蛋白家族:

Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.

The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity.

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

研究领域

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). Quercetin-induced pyroptosis in colon cancer through NEK7-mediated NLRP3 inflammasome-GSDMD signaling pathway activation. American journal of cancer research, 2024 (PubMed: 38590424) [IF=5.3]

Application: WB    Species: Human    Sample: colon cancer tissues

Figure 7 Downregulated expression of NEK7 in human colon cancer tissues and correlated with NLRP3 expression. A. Gene correlation between NLRP3 and NEK7 in colon cancer investigated using the TIMER2.0 database based on Spearman’s correlation analysis. Rho represents the correlation index. B. Influence of NEK7 knockdown on NLRP3 protein expression determined using western blotting (n=3, where “n” represents the number of independent repeated experiments). Values are presented as mean ± SD. *P
2). Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35498125)

Application: WB    Species: Rat    Sample: diabetic myocardium

"Figure 4 SG attenuates the activation of NLRP3 in diabetic myocardium. (a) Protein levels of NLRP3-related molecules shown by western blot. β-Tubulin was an internal reference control. n = 3 in each group. (b) Relative mRNA levels of Nlrp3-relaterd genes shown by real-time RT-PCR. Tubb3 served as a reference gene. n = 3 in each group. (c) Ratio of caspase-1 p12/procaspase-1. n = 3 in each group. (d) Caspase-1 activity in heart tissues. n = 6 in each group. Immunohistochemical staining of (e) NLRP3, (f) ASC, (g) GSDMD, (h) IL-1β, (i) IL-18, and (j) NEK7. Scale bar, 50 μm. (k) Double-labeling immunofluorescence of NLRP3 and CD68. Scale bar, 50 μm. Data are presented as mean ± SD. ∗∗P < 0.01 CON vs. SHAM; ##P < 0.01 SG vs. SHAM. n = 3 in each group."

Application: IHC    Species: Rat    Sample: diabetic myocardium

"Figure 4 SG attenuates the activation of NLRP3 in diabetic myocardium. (a) Protein levels of NLRP3-related molecules shown by western blot. β-Tubulin was an internal reference control. n = 3 in each group. (b) Relative mRNA levels of Nlrp3-relaterd genes shown by real-time RT-PCR. Tubb3 served as a reference gene. n = 3 in each group. (c) Ratio of caspase-1 p12/procaspase-1. n = 3 in each group. (d) Caspase-1 activity in heart tissues. n = 6 in each group. Immunohistochemical staining of (e) NLRP3, (f) ASC, (g) GSDMD, (h) IL-1β, (i) IL-18, and (j) NEK7. Scale bar, 50 μm. (k) Double-labeling immunofluorescence of NLRP3 and CD68. Scale bar, 50 μm. Data are presented as mean ± SD. ∗∗P < 0.01 CON vs. SHAM; ##P < 0.01 SG vs. SHAM. n = 3 in each group."

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