产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4470, RRID:AB_2836825.
展开/折叠
hPlk2; hSNK; PLK-2; Plk2; PLK2_HUMAN; Polo like kinase 2; Polo-like kinase 2; Serine/threonine protein kinase PLK2; Serine/threonine protein kinase SNK; Serine/threonine-protein kinase PLK2; Serine/threonine-protein kinase SNK; Serum Induced Kinase; Serum-inducible kinase; SNK;
抗原和靶标
- Q9NYY3 PLK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELLRTITYQPAASTKMCEQALGKGCGADSKKKRPPQPPEESQPPQSQAQVPPAAPHHHHHHSHSGPEISRIIVDPTTGKRYCRGKVLGKGGFAKCYEMTDLTNNKVYAAKIIPHSRVAKPHQREKIDKEIELHRILHHKHVVQFYHYFEDKENIYILLEYCSRRSMAHILKARKVLTEPEVRYYLRQIVSGLKYLHEQEILHRDLKLGNFFINEAMELKVGDFGLAARLEPLEHRRRTICGTPNYLSPEVLNKQGHGCESDIWALGCVMYTMLLGRPPFETTNLKETYRCIREARYTMPSSLLAPAKHLIASMLSKNPEDRPSLDDIIRHDFFLQGFTPDRLSSSCCHTVPDFHLSSPAKNFFKKAAAALFGGKKDKARYIDTHNRVSKEDEDIYKLRHDLKKTSITQQPSKHRTDEELQPPTTTVARSGTPAVENKQQIGDAIRMIVRGTLGSCSSSSECLEDSTMGSVADTVARVLRGCLENMPEADCIPKEQLSTSFQWVTKWVDYSNKYGFGYQLSDHTVGVLFNNGAHMSLLPDKKTVHYYAELGQCSVFPATDAPEQFISQVTVLKYFSHYMEENLMDGGDLPSVTDIRRPRLYLLQWLKSDKALMMLFNDGTFQVNFYHDHTKIIICSQNEEYLLTYINEDRISTTFRLTTLLMSGCSSELKNRMEYALNMLLQRCN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NYY3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T6 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| C26 | S-Nitrosylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| T100 | Phosphorylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| Y271 | Phosphorylation | Uniprot | |
| Y297 | Phosphorylation | Uniprot | |
| T298 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| S301 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| S302 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| S313 | Phosphorylation | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| S324 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| K366 | Methylation | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| S389 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| K390 | Ubiquitination | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| T416 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| K438 | Ubiquitination | Uniprot | |
| S500 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| S591 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
| T593 | Phosphorylation | Q9NYY3 (PLK2) | Uniprot |
翻译修饰 - Q9NYY3 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O15350 (TP73) | S48 | Uniprot |
| O43166 (SIPA1L1) | S1349 | Uniprot |
| O43852 (CALU) | S44 | Uniprot |
| O43852 (CALU) | T60 | Uniprot |
| O43852 (CALU) | T196 | Uniprot |
| P06748 (NPM1) | S4 | Uniprot |
| P37840 (SNCA) | S129 | Uniprot |
| P53350 (PLK1) | S137 | Uniprot |
| P62258 (YWHAE) | T208 | Uniprot |
| Q16143 (SNCB) | S118 | Uniprot |
| Q969H0 (FBXW7) | S25 | Uniprot |
| Q969H0 (FBXW7) | S176 | Uniprot |
| Q969H0 (FBXW7) | S349 | Uniprot |
| Q9HC77 (CENPJ) | S589 | Uniprot |
| Q9HC77 (CENPJ) | S595 | Uniprot |
| Q9NYY3 (PLK2) | T298 | Uniprot |
| Q9NYY3 (PLK2) | S301 | Uniprot |
| Q9NYY3 (PLK2) | S302 | Uniprot |
| Q9NYY3 (PLK2) | S324 | Uniprot |
| Q9NYY3 (PLK2) | S389 | Uniprot |
| Q9NYY3 (PLK2) | T416 | Uniprot |
| Q9NYY3 (PLK2) | S500 | Uniprot |
| Q9NYY3 (PLK2) | S591 | Uniprot |
| Q9NYY3 (PLK2) | T593 | Uniprot |
研究背景
Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress.
Catalytic activity is enhanced by phosphorylation of Thr-239.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cell projection>Dendrite.
Note: Localizes to centrosomes during early G1 phase where it only associates to the mother centriole and then distributes equally to both mother and daughter centrioles at the onset of S phase.
Expressed at higher level in the fetal lung, kidney, spleen and heart.
Interacts with NSF; causing NSF dissociation from GRIA2. Interacts with CIB1 (By similarity).
The POLO box domains act as phosphopeptide-binding module that recognize and bind serine-[phosphothreonine/phosphoserine]-(proline/X) motifs. PLK2 recognizes and binds docking proteins that are already phosphorylated on these motifs, and then phosphorylates them (By similarity).
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
研究领域
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
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