产品: PLK3 抗体
货号: DF4471
描述: Rabbit polyclonal antibody to PLK3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 70 KD; 72kD(Calculated).
蛋白号: Q9H4B4
RRID: AB_2836826

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(91%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
PLK3 Antibody detects endogenous levels of total PLK3.
RRID:
AB_2836826
引用格式: Affinity Biosciences Cat# DF4471, RRID:AB_2836826.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CNK; Cytokine Inducible Kinase; Cytokine-inducible serine/threonine-protein kinase; FGF inducible kinase; FGF-inducible kinase; FNK; PLK-3; Plk3; PLK3_HUMAN; Polo Like Kinase 3; Polo-like kinase 3; PRK; Proliferation Related Kinase; Proliferation-related kinase; Serine/threonine-protein kinase PLK3;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9H4B4 PLK3_HUMAN:

Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.

序列:
MEPAAGFLSPRPFQRAAAAPAPPAGPGPPPSALRGPELEMLAGLPTSDPGRLITDPRSGRTYLKGRLLGKGGFARCYEATDTETGSAYAVKVIPQSRVAKPHQREKILNEIELHRDLQHRHIVRFSHHFEDADNIYIFLELCSRKSLAHIWKARHTLLEPEVRYYLRQILSGLKYLHQRGILHRDLKLGNFFITENMELKVGDFGLAARLEPPEQRKKTICGTPNYVAPEVLLRQGHGPEADVWSLGCVMYTLLCGSPPFETADLKETYRCIKQVHYTLPASLSLPARQLLAAILRASPRDRPSIDQILRHDFFTKGYTPDRLPISSCVTVPDLTPPNPARSLFAKVTKSLFGRKKKSKNHAQERDEVSGLVSGLMRTSVGHQDARPEAPAASGPAPVSLVETAPEDSSPRGTLASSGDGFEEGLTVATVVESALCALRNCIAFMPPAEQNPAPLAQPEPLVWVSKWVDYSNKFGFGYQLSSRRVAVLFNDGTHMALSANRKTVHYNPTSTKHFSFSVGAVPRALQPQLGILRYFASYMEQHLMKGGDLPSVEEVEVPAPPLLLQWVKTDQALLMLFSDGTVQVNFYGDHTKLILSGWEPLLVTFVARNRSACTYLASHLRQLGCSPDLRQRLRYALRLLRDRSPA

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Xenopus
91
Zebrafish
91
Chicken
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9H4B4 作为底物

Site PTM Type Enzyme
Y136 Phosphorylation
T194 Phosphorylation
T348 Phosphorylation Q9H4B4 (PLK3)
S433 Phosphorylation
T503 Phosphorylation
Y506 Phosphorylation
S510 Phosphorylation
S515 Phosphorylation
T614 Phosphorylation
Y615 Phosphorylation

翻译修饰 - Q9H4B4 作为激酶

Substrate Site Source
O43852 (CALU) S44 Uniprot
O43852 (CALU) T60 Uniprot
O43852 (CALU) T177 Uniprot
O43852 (CALU) T196 Uniprot
O43852 (CALU) T254 Uniprot
O96017 (CHEK2) S62 Uniprot
O96017-12 (CHEK2) T68 Uniprot
O96017 (CHEK2) S73 Uniprot
P04637 (TP53) S20 Uniprot
P05412 (JUN) S63 Uniprot
P05412 (JUN) S73 Uniprot
P06748 (NPM1) S4 Uniprot
P08238 (HSP90AB1) S718 Uniprot
P11388 (TOP2A) T1343 Uniprot
P14635 (CCNB1) S133 Uniprot
P15336 (ATF2) T69 Uniprot
P15336 (ATF2) T71 Uniprot
P30304 (CDC25A) T80 Uniprot
P30304 (CDC25A) S513 Uniprot
P30304 (CDC25A) S519 Uniprot
P30307 (CDC25C) S191 Uniprot
P30307 (CDC25C) S198 Uniprot
P30307 (CDC25C) S216 Uniprot
P37840 (SNCA) S129 Uniprot
P60484 (PTEN) T366 Uniprot
P60484 (PTEN) S370 Uniprot
P62258 (YWHAE) T208 Uniprot
Q07817 (BCL2L1) S49 Uniprot
Q16143 (SNCB) S118 Uniprot
Q16665 (HIF1A) S576 Uniprot
Q16665 (HIF1A) S657 Uniprot
Q99986 (VRK1) S342 Uniprot
Q9H4B4 (PLK3) T348 Uniprot

研究背景

功能:

Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.

翻译修饰:

Phosphorylated in an ATM-dependent manner following DNA damage. Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.

细胞定位:

Cytoplasm. Nucleus. Nucleus>Nucleolus. Golgi apparatus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Translocates to the nucleus upon cisplatin treatment. Localizes to the Golgi apparatus during interphase. According to a report, PLK3 localizes only in the nucleolus and not in the centrosome, or in any other location in the cytoplasm (PubMed:17264206). The discrepancies in results may be explained by the PLK3 antibody specificity, by cell line-specific expression or post-translational modifications.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.

亚基结构:

Interacts (via the POLO-box domain) with CIB1; leading to inhibit PLK3 kinase activity. Interacts with GOLGB1.

蛋白家族:

The POLO box domains act as phosphopeptide-binding module that recognize and bind serine-[phosphothreonine/phosphoserine]-(proline/X) motifs. PLK3 recognizes and binds docking proteins that are already phosphorylated on these motifs, and then phosphorylates them (By similarity). The POLO box domains mediates localization to the centrosome.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.

研究领域

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

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