产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4471, RRID:AB_2836826.
展开/折叠
CNK; Cytokine Inducible Kinase; Cytokine-inducible serine/threonine-protein kinase; FGF inducible kinase; FGF-inducible kinase; FNK; PLK-3; Plk3; PLK3_HUMAN; Polo Like Kinase 3; Polo-like kinase 3; PRK; Proliferation Related Kinase; Proliferation-related kinase; Serine/threonine-protein kinase PLK3;
抗原和靶标
Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
- Q9H4B4 PLK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPAAGFLSPRPFQRAAAAPAPPAGPGPPPSALRGPELEMLAGLPTSDPGRLITDPRSGRTYLKGRLLGKGGFARCYEATDTETGSAYAVKVIPQSRVAKPHQREKILNEIELHRDLQHRHIVRFSHHFEDADNIYIFLELCSRKSLAHIWKARHTLLEPEVRYYLRQILSGLKYLHQRGILHRDLKLGNFFITENMELKVGDFGLAARLEPPEQRKKTICGTPNYVAPEVLLRQGHGPEADVWSLGCVMYTLLCGSPPFETADLKETYRCIKQVHYTLPASLSLPARQLLAAILRASPRDRPSIDQILRHDFFTKGYTPDRLPISSCVTVPDLTPPNPARSLFAKVTKSLFGRKKKSKNHAQERDEVSGLVSGLMRTSVGHQDARPEAPAASGPAPVSLVETAPEDSSPRGTLASSGDGFEEGLTVATVVESALCALRNCIAFMPPAEQNPAPLAQPEPLVWVSKWVDYSNKFGFGYQLSSRRVAVLFNDGTHMALSANRKTVHYNPTSTKHFSFSVGAVPRALQPQLGILRYFASYMEQHLMKGGDLPSVEEVEVPAPPLLLQWVKTDQALLMLFSDGTVQVNFYGDHTKLILSGWEPLLVTFVARNRSACTYLASHLRQLGCSPDLRQRLRYALRLLRDRSPA
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H4B4 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
Y136 | Phosphorylation | Uniprot | |
T194 | Phosphorylation | Uniprot | |
T348 | Phosphorylation | Q9H4B4 (PLK3) | Uniprot |
S433 | Phosphorylation | Uniprot | |
T503 | Phosphorylation | Uniprot | |
Y506 | Phosphorylation | Uniprot | |
S510 | Phosphorylation | Uniprot | |
S515 | Phosphorylation | Uniprot | |
T614 | Phosphorylation | Uniprot | |
Y615 | Phosphorylation | Uniprot |
翻译修饰 - Q9H4B4 作为激酶
Substrate | Site | Source |
---|---|---|
O43852 (CALU) | S44 | Uniprot |
O43852 (CALU) | T60 | Uniprot |
O43852 (CALU) | T177 | Uniprot |
O43852 (CALU) | T196 | Uniprot |
O43852 (CALU) | T254 | Uniprot |
O96017 (CHEK2) | S62 | Uniprot |
O96017-12 (CHEK2) | T68 | Uniprot |
O96017 (CHEK2) | S73 | Uniprot |
P04637 (TP53) | S20 | Uniprot |
P05412 (JUN) | S63 | Uniprot |
P05412 (JUN) | S73 | Uniprot |
P06748 (NPM1) | S4 | Uniprot |
P08238 (HSP90AB1) | S718 | Uniprot |
P11388 (TOP2A) | T1343 | Uniprot |
P14635 (CCNB1) | S133 | Uniprot |
P15336 (ATF2) | T69 | Uniprot |
P15336 (ATF2) | T71 | Uniprot |
P30304 (CDC25A) | T80 | Uniprot |
P30304 (CDC25A) | S513 | Uniprot |
P30304 (CDC25A) | S519 | Uniprot |
P30307 (CDC25C) | S191 | Uniprot |
P30307 (CDC25C) | S198 | Uniprot |
P30307 (CDC25C) | S216 | Uniprot |
P37840 (SNCA) | S129 | Uniprot |
P60484 (PTEN) | T366 | Uniprot |
P60484 (PTEN) | S370 | Uniprot |
P62258 (YWHAE) | T208 | Uniprot |
Q07817 (BCL2L1) | S49 | Uniprot |
Q16143 (SNCB) | S118 | Uniprot |
Q16665 (HIF1A) | S576 | Uniprot |
Q16665 (HIF1A) | S657 | Uniprot |
Q99986 (VRK1) | S342 | Uniprot |
Q9H4B4 (PLK3) | T348 | Uniprot |
研究背景
Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.
Phosphorylated in an ATM-dependent manner following DNA damage. Phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis.
Cytoplasm. Nucleus. Nucleus>Nucleolus. Golgi apparatus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Translocates to the nucleus upon cisplatin treatment. Localizes to the Golgi apparatus during interphase. According to a report, PLK3 localizes only in the nucleolus and not in the centrosome, or in any other location in the cytoplasm (PubMed:17264206). The discrepancies in results may be explained by the PLK3 antibody specificity, by cell line-specific expression or post-translational modifications.
Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor.
Interacts (via the POLO-box domain) with CIB1; leading to inhibit PLK3 kinase activity. Interacts with GOLGB1.
The POLO box domains act as phosphopeptide-binding module that recognize and bind serine-[phosphothreonine/phosphoserine]-(proline/X) motifs. PLK3 recognizes and binds docking proteins that are already phosphorylated on these motifs, and then phosphorylates them (By similarity). The POLO box domains mediates localization to the centrosome.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
研究领域
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
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