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| 产品: | PPP2R5A 抗体 |
| 货号: | DF4482 |
| 描述: | Rabbit polyclonal antibody to PPP2R5A |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 57 KD; 56kD(Calculated). |
| 蛋白号: | Q15172 |
| RRID: | AB_2836833 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4482, RRID:AB_2836833.
展开/折叠
2A5A_HUMAN; B56A; MGC131915; PP2A B subunit B' alpha isoform; PP2A B subunit B56 alpha isoform; PP2A B subunit isoform B''-alpha; PP2A B subunit isoform B56-alpha; PP2A B subunit isoform PR61-alpha; PP2A B subunit isoform R5-alpha; PP2A B subunit PR61 alpha isoform; PP2A B subunit R5 alpha isoform; PPP2R5A; PR61A; PR61alpha; Protein phosphatase 2 regulatory subunit B (B56) alpha isoform; Protein phosphatase 2 regulatory subunit B' alpha; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform;
抗原和靶标
- Q15172 2A5A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSSSPPAGAASAAISASEKVDGFTRKSVRKAQRQKRSQGSSQFRSQGSQAELHPLPQLKDATSNEQQELFCQKLQQCCILFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMISANIFRTLPPSDNPDFDPEEDEPTLEASWPHIQLVYEFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLYKISKEHWNPTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSILSNTSAE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15172 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | P19525 (EIF2AK2) | Uniprot |
| S38 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S42 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| K181 | Acetylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| T421 | Phosphorylation | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| Y474 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | Uniprot |
研究背景
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Phosphorylated on serine residues.
Cytoplasm. Nucleus. Chromosome>Centromere.
Note: From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase.
Widely expressed with the highest expression in heart and skeletal muscle.
PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGO1.
Belongs to the phosphatase 2A regulatory subunit B56 family.
研究领域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Genetic Information Processing > Translation > mRNA surveillance pathway.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
文献引用
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