产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4484, RRID:AB_2836835.
展开/折叠
2AAB_HUMAN; beta isoform of regulatory subunit A, protein phosphatase 2; PP2A A beta; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta; PP2A, subunit A, PR65 beta isoform; PP2A, subunit A, R1 beta isoform; PPP2R1B; PPP4R1; PR65B; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform; protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform; Protein phosphatase 2, regulatory subunit A, beta; Protein phosphatase 2, structural/regulatory subunit A, beta; protein phosphatase 2, structural/regulatory subunit A, beta isoform; serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform;
抗原和靶标
- P30154 2AAB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGASELGTGPGAAGGDGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGNFTGLVGGPDFAHCLLPPLENLATVEETVVRDKAVESLRQISQEHTPVALEAYFVPLVKRLASGDWFTSRTSACGLFSVCYPRASNAVKAEIRQQFRSLCSDDTPMVRRAAASKLGEFAKVLELDSVKSEIVPLFTSLASDEQDSVRLLAVEACVSIAQLLSQDDLETLVMPTLRQAAEDKSWRVRYMVADRFSELQKAMGPKITLNDLIPAFQNLLKDCEAEVRAAAAHKVKELGENLPIEDRETIIMNQILPYIKELVSDTNQHVKSALASVIMGLSTILGKENTIEHLLPLFLAQLKDECPDVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKFGTEWAQNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGQEITTKQMLPIVLKMAGDQVANVRFNVAKSLQKIGPILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVLALA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P30154 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K200 | Acetylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| R278 | Methylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| C402 | S-Nitrosylation | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| K454 | Sumoylation | Uniprot | |
| K484 | Ubiquitination | Uniprot | |
| T493 | Phosphorylation | Uniprot | |
| K497 | Ubiquitination | Uniprot | |
| K539 | Ubiquitination | Uniprot | |
| K554 | Ubiquitination | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| K573 | Sumoylation | Uniprot | |
| K573 | Ubiquitination | Uniprot | |
| K578 | Ubiquitination | Uniprot |
研究背景
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with SGO1. Interacts with RAF1.
Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
Belongs to the phosphatase 2A regulatory subunit A family.
研究领域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Genetic Information Processing > Translation > mRNA surveillance pathway.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Nervous system > Long-term depression.
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