产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4501, RRID:AB_2836852.
展开/折叠
AUTS17; Cortactin binding protein 1; Cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CortBP1; CTTNBP1; GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; KIAA1022; Proline rich synapse associated protein 1; Proline-rich synapse-associated protein 1; ProSAP1; SH3 and multiple ankyrin repeat domains 2; SH3 and multiple ankyrin repeat domains protein 2; SHAN2_RAT; SHANK; Shank2; SPANK-3;
抗原和靶标
- Q9UPX8 SHAN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKSLLNAFTKKEVPFREAPAYSNRRRRPPNTLAAPRVLLRSNSDNNLNASAPDWAVCSTATSHRSLSPQLLQQMPSKPEGAAKTIGSYVPGPRSRSPSLNRLGGAGEDGKRPQPLWHVGSPFALGANKDSLSAFEYPGPKRKLYSAVPGRLFVAVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVQCKPRDSQAETRADRSKKLFRHYTVGSYDSFDTSSDCIIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELVDKASVRKKKDKPEEIVPASKPSRAAENMAVEPRVATIKQRPSSRCFPAGSDMNSVYERQGIAVMTPTVPGSPKAPFLGIPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPKSPTPRVYGTIKPAFNQNSAAKVSPATRSDTVATMMREKGMYFRRELDRYSLDSEDLYSRNAGPQANFRNKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQAPEPPSQLRPDESLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRTRPSMFPEEGDFADEDSAEQLSSPMPSATPREPENHFVGGAEASAPGEAGRPLNSTSKAQGPESSPAVPSASSGTAGPGNYVHPLTGRLLDPSSPLALALSARDRAMKESQQGPKGEAPKADLNKPLYIDTKMRPSLDAGFPTVTRQNTRGPLRRQETENKYETDLGRDRKGDDKKNMLIDIMDTSQQKSAGLLMVHTVDATKLDNALQEEDEKAEVEMKPDSSPSEVPEGVSETEGALQISAAPEPTTVPGRTIVAVGSMEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRAASVPALSDLVKQKKSDTPQSPSLNSSQPTNSADSKKPASLSNCLPASFLPPPESFDAVADSGIEEVDSRSSSDHHLETTSTISTVSSISTLSSEGGENVDTCTVYADGQAFMVDKPPVPPKPKMKPIIHKSNALYQDALVEEDVDSFVIPPPAPPPPPGSAQPGMAKVLQPRTSKLWGDVTEIKSPILSGPKANVISELNSILQQMNREKLAKPGEGLDSPMGAKSASLAPRSPEIMSTISGTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGTRRAPSPVVSPTEMNKETLPAPLSAATASPSPALSDVFSLPSQPPSGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UPX8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S41 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| T84 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| Y88 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| Y144 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| T339 | Phosphorylation | Uniprot | |
| T341 | Phosphorylation | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| T363 | Phosphorylation | Uniprot | |
| T367 | Phosphorylation | Uniprot | |
| S372 | Phosphorylation | Uniprot | |
| Y437 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| Y538 | Phosphorylation | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| S559 | Phosphorylation | Uniprot | |
| T561 | Phosphorylation | Uniprot | |
| T564 | Phosphorylation | Uniprot | |
| Y580 | Phosphorylation | Uniprot | |
| S581 | Phosphorylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| Y588 | Phosphorylation | Uniprot | |
| Y610 | Phosphorylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| Y622 | Phosphorylation | Uniprot | |
| S643 | Phosphorylation | Uniprot | |
| S649 | Phosphorylation | Uniprot | |
| T662 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S725 | Phosphorylation | Uniprot | |
| T726 | Phosphorylation | Uniprot | |
| S813 | Phosphorylation | Uniprot | |
| Y822 | Phosphorylation | Uniprot | |
| T827 | Phosphorylation | Uniprot | |
| S835 | Phosphorylation | Uniprot | |
| Y869 | Phosphorylation | Uniprot | |
| S877 | Phosphorylation | Uniprot | |
| T890 | Phosphorylation | Uniprot | |
| T899 | Phosphorylation | Uniprot | |
| Y903 | Phosphorylation | Uniprot | |
| S931 | Phosphorylation | Uniprot | |
| T939 | Phosphorylation | Uniprot | |
| T943 | Phosphorylation | Uniprot | |
| S1001 | Phosphorylation | Uniprot | |
| S1051 | Phosphorylation | Uniprot | |
| S1064 | Phosphorylation | Uniprot | |
| S1069 | Phosphorylation | Uniprot | |
| S1071 | Phosphorylation | Uniprot | |
| S1080 | Phosphorylation | Uniprot | |
| S1103 | Phosphorylation | Uniprot | |
| S1234 | Phosphorylation | Uniprot | |
| S1269 | Phosphorylation | Uniprot | |
| S1282 | Phosphorylation | Uniprot | |
| S1330 | Phosphorylation | Uniprot | |
| S1334 | Phosphorylation | Uniprot | |
| S1382 | Phosphorylation | Uniprot | |
| S1384 | Phosphorylation | Uniprot | |
| S1386 | Phosphorylation | Uniprot | |
| S1395 | Phosphorylation | Uniprot |
研究背景
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
Apical cell membrane. Cytoplasm. Cell junction>Synapse. Cell junction>Synapse>Postsynaptic density. Cell projection>Growth cone. Cell projection>Dendritic spine.
Note: Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Colocalized with PDE4D to the apical membrane of colonic crypt cells (By similarity).
Isoform 3 is present in epithelial colonic cells (at protein level).
Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with CTTN/cortactin SH3 domain, DLGAP1/GKAP and alpha-latrotoxin receptor 1. Interacts with DNM2, DBNL, GRID2, BAIAP2, SLC9A3, PLCB3 and CFTR. Interacts (via proline-rich region) with PDE4D. Interacts with ABI1 (via SH3 domain).
The PDZ domain is required for interaction with GRID2, PLCB3, CFTR and SLC9A3.
Belongs to the SHANK family.
研究领域
· Organismal Systems > Nervous system > Glutamatergic synapse.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.