| 产品: | SF3B3 抗体 |
| 货号: | DF4539 |
| 描述: | Rabbit polyclonal antibody to SF3B3 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 136 KD; 136kD(Calculated). |
| 蛋白号: | Q15393 |
| RRID: | AB_2836890 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4539, RRID:AB_2836890.
展开/折叠
130 kDa Sin3-associated polypeptide; FLJ12761; Histone deacetylase complex subunit SAP130; KIAA0017; mSin3A associated protein 130; Pre mRNA splicing factor SF3b 130 kDa subunit; RSE1; SAP 130; Sap130; SF3b 130; SF3b130; SF3B3; Sin3-associated polypeptide p130; Sin3A associated protein 130kDa; Sin3A associated protein; SP130_HUMAN; Spliceosome associated protein 130; Splicing factor 3b subunit 3 130kDa; Splicing factor 3b subunit 3; STAF 130; STAF130;
抗原和靶标
- Q15393 SF3B3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPDPNTGKVHTLLTVEVFGVIRSLMAFRLTGGTKDYIVVGSDSGRIVILEYQPSKNMFEKIHQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKLVYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTFYELDLGLNHVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDDPERGMIFVCSATHKTKSMFFFLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVPVAAAMCVLKTGFLFVASEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDELDSLSPILFCQIADLANEDTPQLYVACGRGPRSSLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTGGELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILNPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15393 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K26 | Ubiquitination | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| Y77 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K109 | Acetylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K126 | Acetylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| T153 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| R268 | Methylation | Uniprot | |
| T314 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| S687 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| K700 | Ubiquitination | Uniprot | |
| K769 | Ubiquitination | Uniprot | |
| T784 | Phosphorylation | Uniprot | |
| K787 | Ubiquitination | Uniprot | |
| Y807 | Phosphorylation | Uniprot | |
| K812 | Ubiquitination | Uniprot | |
| K816 | Ubiquitination | Uniprot | |
| Y904 | Phosphorylation | Uniprot | |
| Y926 | Phosphorylation | Uniprot | |
| K936 | Ubiquitination | Uniprot | |
| K942 | Ubiquitination | Uniprot | |
| K965 | Ubiquitination | Uniprot | |
| R968 | Methylation | Uniprot | |
| K974 | Ubiquitination | Uniprot | |
| Y989 | Phosphorylation | Uniprot | |
| Y1029 | Phosphorylation | Uniprot | |
| Y1041 | Phosphorylation | Uniprot | |
| T1043 | Phosphorylation | Uniprot | |
| K1049 | Ubiquitination | Uniprot | |
| K1074 | Ubiquitination | Uniprot | |
| R1079 | Methylation | Uniprot | |
| K1088 | Ubiquitination | Uniprot | |
| R1164 | Methylation | Uniprot | |
| K1171 | Ubiquitination | Uniprot | |
| K1189 | Ubiquitination | Uniprot | |
| K1191 | Ubiquitination | Uniprot | |
| S1194 | Phosphorylation | Uniprot | |
| T1200 | Phosphorylation | Uniprot | |
| K1206 | Acetylation | Uniprot | |
| K1206 | Ubiquitination | Uniprot |
研究背景
Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Nucleus.
Identified in the spliceosome A complex; remains associated with the spliceosome throughout the splicing process. Component of the spliceosome B complex. Identified in the spliceosome C complex. Identified in the spliceosome E complex. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interaction between SF3B3 and SF3B1 is tighter than the interaction between SF3B3 and SF3B2. Within the SF3B complex interacts directly with SF3B1 (via HEAT domain), SF3B5 and PHF5A. The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Associates with the STAGA transcription coactivator-HAT complex. Interacts with SUPT3H. Interacts with TAF3.
The core of the protein consists of three beta-propeller domains.
Belongs to the RSE1 family.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
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