产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4646, RRID:AB_2836937.
展开/折叠
ERIC 1; ERIC-1; ERIC1; MGC117382; MGC133242; OTTHUMP00000113796; TACC3; TACC3_HUMAN; Transforming acidic coiled coil containing protein 3; Transforming acidic coiled-coil-containing protein 3;
抗原和靶标
- Q9Y6A5 TACC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLQVLNDKNVSNEKNTENCDFLFSPPEVTGRSSVLRVSQKENVPPKNLAKAMKVTFQTPLRDPQTHRILSPSMASKLEAPFTQDDTLGLENSHPVWTQKENQQLIKEVDAKTTHGILQKPVEADTDLLGDASPAFGSGSSSESGPGALADLDCSSSSQSPGSSENQMVSPGKVSGSPEQAVEENLSSYSLDRRVTPASETLEDPCRTESQHKAETPHGAEEECKAETPHGAEEECRHGGVCAPAAVATSPPGAIPKEACGGAPLQGLPGEALGCPAGVGTPVPADGTQTLTCAHTSAPESTAPTNHLVAGRAMTLSPQEEVAAGQMASSSRSGPVKLEFDVSDGATSKRAPPPRRLGERSGLKPPLRKAAVRQQKAPQEVEEDDGRSGAGEDPPMPASRGSYHLDWDKMDDPNFIPFGGDTKSGCSEAQPPESPETRLGQPAAEQLHAGPATEEPGPCLSQQLHSASAEDTPVVQLAAETPTAESKERALNSASTSLPTSCPGSEPVPTHQQGQPALELKEESFRDPAEVLGTGAEVDYLEQFGTSSFKESALRKQSLYLKFDPLLRDSPGRPVPVATETSSMHGANETPSGRPREAKLVEFDFLGALDIPVPGPPPGVPAPGGPPLSTGPIVDLLQYSQKDLDAVVKATQEENRELRSRCEELHGKNLELGKIMDRFEEVVYQAMEEVQKQKELSKAEIQKVLKEKDQLTTDLNSMEKSFSDLFKRFEKQKEVIEGYRKNEESLKKCVEDYLARITQEGQRYQALKAHAEEKLQLANEEIAQVRSKAQAEALALQASLRKEQMRIQSLEKTVEQKTKENEELTRICDDLISKMEKI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y6A5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| T30 | Phosphorylation | Uniprot | |
| R32 | Methylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| K51 | Methylation | Uniprot | |
| K54 | Methylation | Uniprot | |
| T56 | Phosphorylation | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| T66 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| T196 | Phosphorylation | Uniprot | |
| C206 | S-Nitrosylation | Uniprot | |
| T216 | Phosphorylation | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Uniprot | |
| Y403 | Phosphorylation | Uniprot | |
| K423 | Acetylation | Uniprot | |
| C426 | S-Nitrosylation | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| T437 | Phosphorylation | Uniprot | |
| T481 | Phosphorylation | Uniprot | |
| T496 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| T534 | Phosphorylation | Uniprot | |
| Y540 | Phosphorylation | Uniprot | |
| K550 | Ubiquitination | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| S558 | Phosphorylation | O14965 (AURKA) | Uniprot |
| Y560 | Phosphorylation | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| T590 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| K649 | Ubiquitination | Uniprot | |
| K668 | Ubiquitination | Uniprot | |
| K674 | Ubiquitination | Uniprot | |
| Y684 | Phosphorylation | Uniprot | |
| K703 | Ubiquitination | Uniprot | |
| K720 | Ubiquitination | Uniprot | |
| S723 | Phosphorylation | Uniprot | |
| Y739 | Phosphorylation | Uniprot | |
| K748 | Ubiquitination | Uniprot | |
| K768 | Ubiquitination | Uniprot | |
| K788 | Ubiquitination | Uniprot | |
| S799 | Phosphorylation | Uniprot | |
| K819 | Ubiquitination | Uniprot | |
| K834 | Ubiquitination | Uniprot |
研究背景
Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. May be involved in the control of cell growth and differentiation. May contribute to cancer.
Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Spindle pole.
Note: In complex with CKAP5 localized to microtubule plus-ends in mitosis and interphase. In complex with CKAP5 and clathrin localized to inter-microtubule bridges in mitotic spindles.
Interacts with microtubules. Interacts with CKAP5 independently of clathrin. Interacts with CKAP5 and clathrin forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges; TACC3 (phosphorylated at Ser-558 by AURKA) and CLTC are proposed to form a composite microtubule interaction surface. Interacts with CCDC100/CEP120. The coiled coil C-terminal region interacts with AH receptor nuclear translocator protein (ARNT) and ARNT2 (By similarity). Interacts with GCN5L2 and PCAF.
Belongs to the TACC family.
研究领域
· Genetic Information Processing > Translation > RNA transport.
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