| 产品: | UGDH 抗体 |
| 货号: | DF4603 |
| 描述: | Rabbit polyclonal antibody to UGDH |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 55 KD; 55kD(Calculated). |
| 蛋白号: | O60701 |
| RRID: | AB_2836967 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4603, RRID:AB_2836967.
展开/折叠
GDH; UDP Glc dehydrogenase; UDP GlcDH; UDP glucose 6 dehydrogenase; UDP-Glc dehydrogenase; UDP-GlcDH; UDP-glucose 6-dehydrogenase; UDP-glucose dehydrogenase; UDPGDH; UGD; Ugdh; UGDH_HUMAN; Uridine diphospho glucose dehydrogenase;
抗原和靶标
Detected in heart, placenta, liver, pancreas, spleen, thymus, prostate, ovary, small intestine and colon (PubMed:9737970). Widely expressed (PubMed:9737970).
- O60701 UGDH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFEIKKICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPTLPIYEPGLKEVVESCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDRVLIGGDETPEGQRAVQALCAVYEHWVPREKILTTNTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVIDMNDYQRRRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDPKVPREQIVVDLSHPGVSEDDQVSRLVTISKDPYEACDGAHAVVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDGLHNELQTIGFQIETIGKKVSSKRIPYAPSGEIPKFSLQDPPNKKPKV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O60701 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S40 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| Y53 | Phosphorylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K107 | Acetylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K173 | Acetylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| T185 | Phosphorylation | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| Y286 | Phosphorylation | Uniprot | |
| Y309 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| K339 | Acetylation | Uniprot | |
| Y352 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K434 | Acetylation | Uniprot | |
| T461 | Phosphorylation | P06493 (CDK1) | Uniprot |
| Y473 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| K490 | Acetylation | Uniprot |
研究背景
Catalyzes the formation of UDP-alpha-D-glucuronate, a constituent of complex glycosaminoglycans. Required for the biosynthesis of chondroitin sulfate and heparan sulfate. Required for embryonic development via its role in the biosynthesis of glycosaminoglycans (By similarity).
Detected in heart, placenta, liver, pancreas, spleen, thymus, prostate, ovary, small intestine and colon. Widely expressed.
Homohexamer.
The protein goes through several conformation states during the reaction cycle, giving rise to hysteresis. In the initial state, the ligand-free protein is in an inactive conformation (E*). Substrate binding triggers a change to the active conformation (E). UDP-xylose binding triggers the transition to a distinct, inhibited conformation. The presence of an intrinsically disordered C-terminus promotes a more dynamic protein structure and favors a conformation with high affinity for UPD-xylose.
The allosteric switch region moves by about 5 Angstroms when UDP-xylose is bound, and occupies part of the UDP-glucose binding site. At the same time it promotes domain movements that disrupt the active hexameric ring structure and lead to the formation of a horseshoe-shaped, inactive hexamer.
Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.
研究领域
· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.
· Metabolism > Carbohydrate metabolism > Ascorbate and aldarate metabolism.
· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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