| 产品: | WIPF1 抗体 |
| 货号: | DF4616 |
| 描述: | Rabbit polyclonal antibody to WIPF1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 52 KD; 51kD(Calculated). |
| 蛋白号: | O43516 |
| RRID: | AB_2836980 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4616, RRID:AB_2836980.
展开/折叠
AI115543; D2Ertd120e; MGC111041; Protein PRPL-2; PRPL 2; PRPL-2 protein; WAS/WASL interacting protein family member 1; WAS/WASL-interacting protein family member 1; WAS2; WASP-interacting protein; WASPIP; WIP; Wipf1; WIPF1_HUMAN; Wiskott-Aldrich syndrome protein interacting protein; Wiskott-Aldrich syndrome protein-interacting protein;
抗原和靶标
Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.
- O43516 WIPF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPVPPPPAPPPPPTFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGAGGGGGGFGGGGGFGGGGGGGGGGSFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPLLPPGGRSTSAKPFSPPSGPGRFPVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSPHNRGSPPVPGGPRQPSPGPTPPPFPGNRGTALGGGSIRQSPLSSSSPFSNRPPLPPTPSRALDDKPPPPPPPVGNRPSIHREAVPPPPPQNNKPPVPSTPRPSASSQAPPPPPPPSRPGPPPLPPSSSGNDETPRLPQRNLSLSSSTPPLPSPGRSGPLPPPPSERPPPPVRDPPGRSGPLPPPPPVSRNGSTSRALPATPQLPSRSGVDSPRSGPRPPLPPDRPSAGAPPPPPPSTSIRNGFQDSPCEDEWESRFYFHPISDLPPPEPYVQTTKSYPSKLARNESRSGSNRRERGAPPLPPIPR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O43516 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R33 | Methylation | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| R125 | Methylation | Uniprot | |
| R134 | Methylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| R149 | Methylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| R211 | Methylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| R226 | Methylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| R236 | Methylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| R249 | Methylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| R258 | Methylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T398 | Phosphorylation | Uniprot | |
| R404 | Methylation | Uniprot | |
| R415 | Methylation | Uniprot | |
| R422 | Methylation | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| Y455 | Phosphorylation | Uniprot | |
| Y468 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Q04759 (PRKCQ) | Uniprot |
| R493 | Methylation | Uniprot |
研究背景
Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation. Plays a role in the formation of cell ruffles (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.
Cytoplasmic vesicle. Cytoplasm>Cytoskeleton. Cell projection>Ruffle.
Note: Vesicle surfaces and along actin tails. Colocalizes with actin stress fibers. When coexpressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL (By similarity).
Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.
Binds to WAS, profilin and actin. Binds to WASL (By similarity). Interacts with DBNL. Interacts with FNBP1L (via the SH3 domain).
Binds to WAS within the N-terminal region 170, at a site distinct from the CDC42-binding site.
Belongs to the verprolin family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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