产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4618, RRID:AB_2836982.
展开/折叠
DICTYOSTELIUM; dJ393P12.2; IMD2; Protein WAVE-2; Putative Wiskott Aldrich syndrome protein family member 4; SCAR 2; SCAR; SCAR2; Suppressor of cyclic-AMP receptor (WASP family); Verprolin homology domain-containing protein 2; WASF2; WASF2_HUMAN; WASF4; WASP family protein member 2; WASP family protein member 4; WASP family Verprolin homologous protein 2; WASP-family protein member 2; WAVE 2; WAVE2; Wiskott-Aldrich syndrome protein family member 2; Wiskott-Aldrich syndrome protein family verprolin-homologous protein;
抗原和靶标
Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.
- Q9Y6W5 WASF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSKYAEDIFGELFTQANTFASRVSSLAERVDRLQVKVTQLDPKEEEVSLQGINTRKAFRSSTIQDQKLFDRNSLPVPVLETYNTCDTPPPLNNLTPYRDDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKLGTSGYPPTLVYQNGSIGCVENVDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPVDNQRGSGLAGPKRSSVVSPSHPPPAPPLGSPPGPKPGFAPPPAPPPPPPPMIGIPPPPPPVGFGSPGTPPPPSPPSFPPHPDFAAPPPPPPPPAADYPTLPPPPLSQPTGGAPPPPPPPPPPGPPPPPFTGADGQPAIPPPLSDTTKPKSSLPAVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEFDEDDWSD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y6W5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T17 | Phosphorylation | Uniprot | |
| T80 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| T137 | Phosphorylation | Uniprot | |
| Y139 | Phosphorylation | Uniprot | |
| Y150 | Phosphorylation | A0A173G4P4 (Abl fusion) , P00519 (ABL1) | Uniprot |
| Y155 | Phosphorylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| Y230 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S298 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S343 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| T346 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S351 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S429 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | Uniprot | |
| T471 | Phosphorylation | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S482 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S484 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S488 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S489 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S497 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
研究背景
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium.
Note: At the interface between the lamellipodial actin meshwork and the membrane.
Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.
Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 (NCKAP1l/HEM1 in hematopoietic cells) and WASF2/WAVE2. Directly interacts with BRK1. Interacts with FNBP1L (via the SH3 domain).
(Microbial infection) Interacts with human cytomegalovirus protein UL135.
Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain.
Belongs to the SCAR/WAVE family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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