产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4625, RRID:AB_2836989.
展开/折叠
Abscission/NoCut checkpoint regulator; ANCHR; MLL partner containing FYVE domain; MPFYVE; ZFY19_HUMAN; ZFYVE 19; Zfyve19; Zinc finger FYVE domain containing protein 19; Zinc finger FYVE domain-containing protein 19; Zinc finger FYVE type containing 19; Zinc finger, FYVE domain containing 19;
抗原和靶标
- Q96K21 ANCHR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNYDSQQPPLPPLPYAGCRRASGFPALGRGGTVPVGVWGGAGQGREGRSWGEGPRGPGLGRRDLSSADPAVLGATMESRCYGCAVKFTLFKKEYGCKNCGRAFCSGCLSFSAAVPRTGNTQQKVCKQCHEVLTRGSSANASKWSPPQNYKKRVAALEAKQKPSTSQSQGLTRQDQMIAERLARLRQENKPKLVPSQAEIEARLAALKDERQGSIPSTQEMEARLAALQGRVLPSQTPQPAHHTPDTRTQAQQTQDLLTQLAAEVAIDESWKGGGPAASLQNDLNQGGPGSTNSKRQANWSLEEEKSRLLAEAALELREENTRQERILALAKRLAMLRGQDPERVTLQDYRLPDSDDDEDEETAIQRVLQQLTEEASLDEASGFNIPAEQASRPWTQPRGAEPEAQDVDPRPEAEEEELPWCCICNEDATLRCAGCDGDLFCARCFREGHDAFELKEHQTSAYSPPRAGQEH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96K21 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y3 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| Y15 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K207 | Sumoylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| T217 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| Y349 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| T459 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot |
研究背景
Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cleavage furrow. Midbody>Midbody ring.
Note: Localizes mainly on centrosomes in interphase and early mitosis. Localizes at the cleavage furrow and midbody ring in late mitosis and cytokinesis.
Detected in brain, heart, skeletal muscle, kidney and liver.
Interacts (via MIM1-B) with VPS4A; interaction takes place at the midbody ring following cytokinesis checkpoint activation.
The FYVE-type zinc finger mediates binding to phosphatidylinositol-3-phosphate (PtdIns(3)P).
The MIM1-B motif mediates interaction with VPS4A.
限制条款
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