产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4678, RRID:AB_2837029.
展开/折叠
E74-like factor 4; ELF4; ELF4_HUMAN; ELFR; ETS-related transcription factor Elf-4; ETS-related transcription factor Elf4; MEF; Myeloid Elf-1-like factor; Myeloid Elf1-like factor;
抗原和靶标
Abundantly expressed in the placenta and in a variety of myeloid leukemia cell lines. Moderate levels of expression in heart, lung, spleen, thymus, peripheral blood lymphocytes, ovary and colon. Lower levels of expression in Jurkat T-cells and other T-cell lines and no expression in brain.
- Q99607 ELF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAITLQPSDLIFEFASNGMDDDIHQLEDPSVFPAVIVEQVPYPDLLHLYSGLELDDVHNGIITDGTLCMTQDQILEGSFLLTDDNEATSHTMSTAEVLLNMESPSDILDEKQIFSTSEMLPDSDPAPAVTLPNYLFPASEPDALNRAGDTSDQEGHSLEEKASREESAKKTGKSKKRIRKTKGNRSTSPVTDPSIPIRKKSKDGKGSTIYLWEFLLALLQDRNTCPKYIKWTQREKGIFKLVDSKAVSKLWGKQKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKEMPKDLVVIEDEDESSEATAAPPQASTASVASASTTRRTSSRVSSRSAPQGKGSSSWEKPKIQHVGLQPSASLELGPSLDEEIPTTSTMLVSPAEGQVKLTKAVSASSVPSNIHLGVAPVGSGSALTLQTIPLTTVLTNGPPASTTAPTQLVLQSVPAASTFKDTFTLQASFPLNASFQDSQVAAPGAPLILSGLPQLLAGANRPTNPAPPTVTGAGPAGPSSQPPGTVIAAFIRTSGTTAAPRVKEGPLRSSSYVQGMVTGAPMEGLLVPEETLRELLRDQAHLQPLPTQVVSRGSHNPSLLGNQTLSPPSRPTVGLTPVAELELSSGSGSLLMAEPSVTTSGSLLTRSPTPAPFSPFNPTSLIKMEPHDI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99607 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K111 | Sumoylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| Y262 | Phosphorylation | Uniprot | |
| R270 | Methylation | Uniprot | |
| Y272 | Phosphorylation | Uniprot | |
| Y273 | Phosphorylation | Uniprot | |
| K280 | Sumoylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| T527 | Phosphorylation | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| S641 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| T643 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S648 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K657 | Sumoylation | Uniprot |
研究背景
Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2 (By similarity). Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway.
Nucleus>PML body.
Note: Accumulation into PML nuclear bodies is mediated by PML.
Abundantly expressed in the placenta and in a variety of myeloid leukemia cell lines. Moderate levels of expression in heart, lung, spleen, thymus, peripheral blood lymphocytes, ovary and colon. Lower levels of expression in Jurkat T-cells and other T-cell lines and no expression in brain.
Interacts with RUNX1 (via the Runt domain); the interaction transactivates the IL3 promoter. Interacts (via its C-terminus) with PML; the interaction translocates ELF4 to PML nuclear bodies and enhances transactivation of LYZ.
Belongs to the ETS family.
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