DUSP10 Antibody - #DF4694

>>买2送1 进行中>>
>>买赠内参/二抗 进行中>>
(2)   (6)  
产品: DUSP10 抗体
货号: DF4694
描述: Rabbit polyclonal antibody to DUSP10
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 52 KD; 53kD(Calculated).
蛋白号: Q9Y6W6
RRID: AB_2837045

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
DUSP10 Antibody detects endogenous levels of total DUSP10.
RRID:
AB_2837045
引用格式: Affinity Biosciences Cat# DF4694, RRID:AB_2837045.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Dual specificity phosphatase 10; Dual specificity phosphatase MKP 5; Dual specificity phosphatase MKP5; Dual specificity protein phosphatase 10; DUS10_HUMAN; DUSP 10; DUSP10; DUSP10 protein; MAP kinase phosphatase 5; Mitogen activated protein kinase phosphatase 5; Mitogen-activated protein kinase phosphatase 5; MKP 5; MKP-5; MKP5; Serine/threonine specific protein phosphatase;

抗原和靶标

免疫原:
Uniprot:

Q9Y6W6(DUS10_HUMAN) >>Visit HPA database.

基因/基因ID:
DUSP10(11221)
表达:
Q9Y6W6 DUS10_HUMAN:

Expressed in keratinocytes (at protein level) (PubMed:29043977). Detected in brain (PubMed:16806267).

序列:
MPPSPLDDRVVVALSRPVRPQDLNLCLDSSYLGSANPGSNSHPPVIATTVVSLKAANLTYMPSSSGSARSLNCGCSSASCCTVATYDKDNQAQTQAIAAGTTTTAIGTSTTCPANQMVNNNENTGSLSPSSGVGSPVSGTPKQLASIKIIYPNDLAKKMTKCSKSHLPSQGPVIIDCRPFMEYNKSHIQGAVHINCADKISRRRLQQGKITVLDLISCREGKDSFKRIFSKEIIVYDENTNEPSRVMPSQPLHIVLESLKREGKEPLVLKGGLSSFKQNHENLCDNSLQLQECREVGGGASAASSLLPQPIPTTPDIENAELTPILPFLFLGNEQDAQDLDTMQRLNIGYVINVTTHLPLYHYEKGLFNYKRLPATDSNKQNLRQYFEEAFEFIEEAHQCGKGLLIHCQAGVSRSATIVIAYLMKHTRMTMTDAYKFVKGKRPIISPNLNFMGQLLEFEEDLNNGVTPRILTPKLMGVETVV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y6W6 作为底物

Site PTM Type Enzyme
S4 Phosphorylation
S224 Phosphorylation P42345 (MTOR)
S230 Phosphorylation P42345 (MTOR)
Y236 Phosphorylation
T240 Phosphorylation
K380 Ubiquitination

研究背景

功能:

Protein phosphatase involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily. It preferably dephosphorylates p38.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in keratinocytes (at protein level). Detected in brain.

亚基结构:

Monomer. Interacts with MAPK14.

蛋白家族:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

文献引用

1). Oroxylin A alleviates myocardial ischemia-reperfusion injury by quelling ferroptosis via activating the DUSP10/MAPK-Nrf2 pathway. Phytotherapy research : PTR, 2024 (PubMed: 39225191) [IF=6.1]
2). DUSP10 is a novel immune-related biomarker connected with survival and cellular proliferation in lower-grade glioma. Aging (Albany NY), 2023 (PubMed: 37387540) [IF=3.9]

Application: WB    Species: Human    Sample: LGG tissues

Figure 7 In vitro experiments verification of DUSP10 in LGG. (A) Western blot analysis of DUSP10 expression in LGG tissues and corresponding para-carcinoma tissues. (B) Western blot and (C) qRT-PCR analysis of DUSP10 expression in NHA and LGG cell lines. (D) The cell viability of si-DUSP10-transfected and si-NC-transfected SW1088 cells by CCK-8 assays. (E, F) Effect of DUSP10 knockdown on colony formation was counted in SW1088 cells. (G, H) Representative images (G) and histogram analysis (H) of EdU assays after DUSP10 knockdown in SW1088 cells. (I, J) Cell cycle assays were implemented to evaluate the cell distribution of the SW1088 cell lines transfected with si-DUSP10 or si-NC lentiviruses.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.