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| 产品: | IGF2BP2 抗体 |
| 货号: | DF4710 |
| 描述: | Rabbit polyclonal antibody to IGF2BP2 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 65 KD; 66kD(Calculated). |
| 蛋白号: | Q9Y6M1 |
| RRID: | AB_2837061 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4710, RRID:AB_2837061.
展开/折叠
Hepatocellular carcinoma autoantigen p62; IF2B2_HUMAN; IGF 2 mRNA binding protein 2; IGF II mRNA binding protein 2; IGF-II mRNA-binding protein 2; IGF2 mRNA-binding protein 2; Igf2bp2; IMP 2; IMP-2; IMP2; Insulin like growth factor 2 mRNA binding protein 2; Insulin-like growth factor 2 mRNA-binding protein 2; OTTHUMP00000082606; OTTHUMP00000082607; OTTHUMP00000082608; p62; VICKZ 2; VICKZ family member 2; VICKZ2;
抗原和靶标
Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
- Q9Y6M1 IF2B2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMNKLYIGNLSPAVTADDLRQLFGDRKLPLAGQVLLKSGYAFVDYPDQNWAIRAIETLSGKVELHGKIMEVDYSVSKKLRSRKIQIRNIPPHLQWEVLDGLLAQYGTVENVEQVNTDTETAVVNVTYATREEAKIAMEKLSGHQFENYSFKISYIPDEEVSSPSPPQRAQRGDHSSREQGHAPGGTSQARQIDFPLRILVPTQFVGAIIGKEGLTIKNITKQTQSRVDIHRKENSGAAEKPVTIHATPEGTSEACRMILEIMQKEADETKLAEEIPLKILAHNGLVGRLIGKEGRNLKKIEHETGTKITISSLQDLSIYNPERTITVKGTVEACASAEIEIMKKLREAFENDMLAVNQQANLIPGLNLSALGIFSTGLSVLSPPAGPRGAPPAAPYHPFTTHSGYFSSLYPHHQFGPFPHHHSYPEQEIVNLFIPTQAVGAIIGKKGAHIKQLARFAGASIKIAPAEGPDVSERMVIITGPPEAQFKAQGRIFGKLKEENFFNPKEEVKLEAHIRVPSSTAGRVIGKGGKTVNELQNLTSAEVIVPRDQTPDENEEVIVRIIGHFFASQTAQRKIREIVQQVKQQEQKYPQGVASQRSK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y6M1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y6 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| T15 | Phosphorylation | Uniprot | |
| Y40 | Phosphorylation | Uniprot | |
| Y45 | Phosphorylation | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| K77 | Methylation | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K78 | Methylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| Y148 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| T223 | Phosphorylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| T269 | Phosphorylation | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| T330 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| K487 | Methylation | Uniprot | |
| K487 | Ubiquitination | Uniprot | |
| K497 | Ubiquitination | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| K509 | Ubiquitination | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| K530 | Ubiquitination | Uniprot | |
| T550 | Phosphorylation | Uniprot | |
| K583 | Ubiquitination | Uniprot | |
| K588 | Ubiquitination | Uniprot |
研究背景
RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation (By similarity). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts.
Nucleus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. In response to cellular stress, such as oxidative stress, recruited to stress granules.
Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
Can form homooligomers and heterooligomers with IGF2BP1 and IGF2BP3 in an RNA-dependent manner. Interacts with HNRPD and IGF2BP1. Interacts with ELAVL1, DHX9 and HNRNPU.
Domain KH3 and KH4 are the major RNA-binding modules, although KH1 and KH2 may also contribute. The contribution to RNA-binding of individual KH domains may be target-specific. KH1 and KH2, and possibly KH3 and KH4, promote the formation of higher ordered protein-RNA complexes, which may be essential for IGF2BP1 cytoplasmic retention. KH domains are required for RNA-dependent homo- and heterooligomerization and for localization to stress granules.
Belongs to the RRM IMP/VICKZ family.
文献引用
Application: WB Species: Human Sample: BEAS-2B cells
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