产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4731, RRID:AB_2837082.
展开/折叠
2510006G12Rik; AA408751; AA536819; AA986414; ANAPC 5; ANAPC5; Anaphase promoting complex subunit 5; Anaphase-promoting complex subunit 5; Apc 5; APC5; APC5_HUMAN; Cyclosome subunit 5; PDL 108; PDL108; Periodontal ligament fibroblast protein; RMC1;
抗原和靶标
- Q9UJX4 APC5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASVHESLYFNPMMTNGVVHANVFGIKDWVTPYKIAVLVLLNEMSRTGEGAVSLMERRRLNQLLLPLLQGPDITLSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQNGEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRSDSYVLLEHSVKKAVHFGLPYLASLGIQSLVQQRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSMNSLEAVNAGVQQNNTESFAVALCHLAELHAEQGCFAAASEVLKHLKERFPPNSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLVTGITALNSIEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSKEYRLQYLASETVLNLAFAQLILGIPEQALSLLHMAIEPILADGAILDKGRAMFLVAKCQVASAASYDQPKKAEALEAAIENLNEAKNYFAKVDCKERIRDVVYFQARLYHTLGKTQERNRCAMLFRQLHQELPSHGVPLINHL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UJX4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T15 | Phosphorylation | Uniprot | |
| T47 | Phosphorylation | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| Y255 | Phosphorylation | Uniprot | |
| Y258 | Phosphorylation | Uniprot | |
| Y277 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| K524 | Ubiquitination | Uniprot | |
| K547 | Ubiquitination | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| K566 | Ubiquitination | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| T598 | Phosphorylation | Uniprot | |
| K669 | Ubiquitination | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| K682 | Ubiquitination | Uniprot | |
| K683 | Ubiquitination | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| K703 | Ubiquitination | Uniprot | |
| K726 | Ubiquitination | Uniprot |
研究背景
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5.
The TPR repeats are six to seven residues longer than a canonical TPR motif.
Belongs to the APC5 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
限制条款
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