| 产品: | RFC2 抗体 |
| 货号: | DF4735 |
| 描述: | Rabbit polyclonal antibody to RFC2 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 39 KD; 39kD(Calculated). |
| 蛋白号: | P35250 |
| RRID: | AB_2837086 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4735, RRID:AB_2837086.
展开/折叠
A1 40 kDa subunit; A1; Activator 1 40 kDa subunit; Activator 1; Activator 1 subunit 2; MGC3665; OTTHUMP00000160645; OTTHUMP00000160646; Replication factor C (activator 1) 2 40kDa; Replication factor C (activator 1) 2; Replication factor C 2; Replication factor C 40 kDa subunit; Replication factor C subunit 2; RF C 40 kDa subunit; RF-C 40 kDa subunit; RFC 2; RFC 40; RFC 40 kDa subunit; RFC2; RFC2_HUMAN; RFC40;
抗原和靶标
- P35250 RFC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVEAVCGGAGEVEAQDSDPAPAFSKAPGSAGHYELPWVEKYRPVKLNEIVGNEDTVSRLEVFAREGNVPNIIIAGPPGTGKTTSILCLARALLGPALKDAMLELNASNDRGIDVVRNKIKMFAQQKVTLPKGRHKIIILDEADSMTDGAQQALRRTMEIYSKTTRFALACNASDKIIEPIQSRCAVLRYTKLTDAQILTRLMNVIEKERVPYTDDGLEAIIFTAQGDMRQALNNLQSTFSGFGFINSENVFKVCDEPHPLLVKEMIQHCVNANIDEAYKILAHLWHLGYSPEDIIGNIFRVCKTFQMAEYLKLEFIKEIGYTHMKIAEGVNSLLQMAGLLARLCQKTMAPVAS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P35250 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| Y34 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| R111 | Methylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| T157 | Phosphorylation | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K163 | Acetylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| K176 | Acetylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| R184 | Methylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| C255 | S-Nitrosylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K304 | Acetylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| K347 | Ubiquitination | Uniprot |
研究背景
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP (By similarity).
Nucleus.
Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA. RFC2 also interacts with PRKAR1A; the complex may be involved in cell survival. Interacts with DDX11.
Belongs to the activator 1 small subunits family.
研究领域
· Genetic Information Processing > Replication and repair > DNA replication.
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Genetic Information Processing > Replication and repair > Mismatch repair.
限制条款
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