PTGIS Antibody - #DF4745

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产品: PTGIS 抗体
货号: DF4745
描述: Rabbit polyclonal antibody to PTGIS
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 57 KD; 57kD(Calculated).
蛋白号: Q16647
RRID: AB_2837096

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(82%), Bovine(100%), Horse(91%), Sheep(100%), Dog(91%)
克隆:
Polyclonal
特异性:
PTGIS Antibody detects endogenous levels of total PTGIS.
RRID:
AB_2837096
引用格式: Affinity Biosciences Cat# DF4745, RRID:AB_2837096.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CYP8; CYP8A1; Cytochrome P450, family 8, subfamily A, polypeptide 1; EC 5.3.99.4; MGC126858; MGC126860; OTTHUMP00000031777; PGIS; Prostacyclin synthase; Prostaglandin I2 (prostacyclin) synthase; Prostaglandin I2 synthase; PTGI; PTGIS; PTGIS_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q16647(PTGIS_HUMAN) >>Visit HPA database.

基因/基因ID:
PTGIS(5740)
表达:
Q16647 PTGIS_HUMAN:

Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate.

序列:
MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMKEKHGDIFTILVGGRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLRLTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNPDGSEKKDFYKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSRYGFGLMQPEHDVPVRYRIRP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Horse
91
Dog
91
Pig
82
Zebrafish
64
Xenopus
56
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q16647 作为底物

Site PTM Type Enzyme
S52 Phosphorylation
S118 Phosphorylation
S200 Phosphorylation
Y398 Phosphorylation
T399 Phosphorylation

研究背景

功能:

Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).

细胞定位:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate.

蛋白家族:

Belongs to the cytochrome P450 family.

研究领域

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Vitamin D stimulates miR-26b-5p to inhibit placental COX-2 expression in preeclampsia. Scientific Reports, 2021 (PubMed: 34045549) [IF=4.6]

Application: IHC    Species: human    Sample: placental

Supplementary Figure 1|. Representative PGIS expression in placenta from normotensive pregnant women and women complicated with preeclampsia (PE). PGIS, prostacyclin synthase, Bar = 100 micron.
2). Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34007408)

Application: WB    Species: Rat    Sample: prostate cell

Figure 6 KLK1 could upregulate NO/cGMP signal and inhibit fibrosis in the prostate of aged rats. (a, b, e, and h) NO content, cGMP content, ADMA content, and cAMP content normalized to total protein concentration of rat prostate samples. (c, f, and i) Representative Western blot results of eNOS, DDAH2, COX-2, PTGIS, TGF-β1, RhoA, and ROCK1 in prostates of all three groups. (d, g, and j) The expression levels of above-mentioned proteins with β-actin as the loading control in all three groups. For each group, values are presented as the mean ± SD of 10 rats per group. ∗P < 0.05, ∗∗P < 0.01 (aTGR vs. aWTR). ##P < 0.01 (aWTR vs. yWTR).

Application: WB    Species: rat    Sample: prostate

Figure 6|: KLK1 could upregulate NO/cGMP signal and inhibit fibrosis in the prostate of aged rats.(c, f, and i) Representative Western blot results of eNOS, DDAH2, COX-2, PTGIS, TGF-β1, RhoA, and ROCK1 in prostates of all three groups.

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