产品: KCNJ4 抗体
货号: DF4765
描述: Rabbit polyclonal antibody to KCNJ4
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Horse, Rabbit, Dog
分子量: 50 KD; 50kD(Calculated).
蛋白号: P48050
RRID: AB_2837119

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Horse(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
KCNJ4 Antibody detects endogenous levels of total KCNJ4.
RRID:
AB_2837119
引用格式: Affinity Biosciences Cat# DF4765, RRID:AB_2837119.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Hippocampal inward rectifier; Hippocampal inward rectifier potassium channel; HIR; HIRK2; HRK1; Inward rectifier K(+) channel Kir2.3; Inward rectifier K+ channel Kir2.3; Inward rectifier potassium channel 4; Inward rectifier potassium channel Kir 2.3; inwardly rectifying subfamily J member 4; IRK-3; IRK3; IRK4_HUMAN; KCNJ 4; Kcnj4; Kir2.3; MGC142066; MGC142068; OTTHUMP00000028922; Potassium channel; Potassium channel inwardly rectifying subfamily J member 4; Potassium inwardly rectifying channel J4; Potassium inwardly rectifying channel subfamily J member 4;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P48050 KCNJ4_HUMAN:

Heart, skeletal muscle, and several different brain regions including the hippocampus.

序列:
MHGHSRNGQAHVPRRKRRNRFVKKNGQCNVYFANLSNKSQRYMADIFTTCVDTRWRYMLMIFSAAFLVSWLFFGLLFWCIAFFHGDLEASPGVPAAGGPAAGGGGAAPVAPKPCIMHVNGFLGAFLFSVETQTTIGYGFRCVTEECPLAVIAVVVQSIVGCVIDSFMIGTIMAKMARPKKRAQTLLFSHHAVISVRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPYMTQEGEYLPLDQRDLNVGYDIGLDRIFLVSPIIIVHEIDEDSPLYGMGKEELESEDFEIVVILEGMVEATAMTTQARSSYLASEILWGHRFEPVVFEEKSHYKVDYSRFHKTYEVAGTPCCSARELQESKITVLPAPPPPPSAFCYENELALMSQEEEEMEEEAAAAAAVAAGLGLEAGSKEEAGIIRMLEFGSHLDLERMQASLPLDNISYRRESAI

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Zebrafish
64
Bovine
0
Sheep
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P48050 作为底物

Site PTM Type Enzyme
T53 Phosphorylation P17252 (PRKCA)
Y234 Phosphorylation P00533 (EGFR)
S356 Phosphorylation
S443 Phosphorylation P17612 (PRKACA)

研究背景

功能:

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity).

细胞定位:

Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cytoplasmic vesicle membrane.
Note: TAX1BP3 binding promotes dissociation of KCNJ4 from LIN7 famaly members and KCNJ4 internalization.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Heart, skeletal muscle, and several different brain regions including the hippocampus.

亚基结构:

Homomultimeric and heteromultimeric association with KCNJ2 and KCNJ12. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking (By similarity). Interacts with TAX1BP3. TAX1BP3 competes with LIN7 family members for KCNJ4 binding.

蛋白家族:

The Val/Gly/Ala/Pro stretch may have a functional role in the conductance or permeation properties.

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ4 subfamily.

研究领域

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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