MRIP Antibody - #DF4766

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产品: MRIP 抗体
货号: DF4766
描述: Rabbit polyclonal antibody to MRIP
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 116 KD; 117kD(Calculated).
蛋白号: Q6WCQ1
RRID: AB_2837120

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
MRIP Antibody detects endogenous levels of total MRIP.
RRID:
AB_2837120
引用格式: Affinity Biosciences Cat# DF4766, RRID:AB_2837120.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

9530046C02; AA536749; AI647711; C76423; KIAA0864; M-RIP; MGC67316; mKIAA0864; Mprip; MPRIP_HUMAN; Myosin phosphatase Rho-interacting protein; p116 Rho-interacting protein; p116Rip; Rho-interacting protein 3; Rhoip3; RIP3; RP23-180B18.4;

抗原和靶标

免疫原:
Uniprot:

Q6WCQ1(MPRIP_HUMAN) >>Visit HPA database.

基因/基因ID:
MPRIP(23164)
序列:
MSAAKENPCRKFQANIFNKSKCQNCFKPRESHLLNDEDLTQAKPIYGGWLLLAPDGTDFDNPVHRSRKWQRRFFILYEHGLLRYALDEMPTTLPQGTINMNQCTDVVDGEGRTGQKFSLCILTPEKEHFIRAETKEIVSGWLEMLMVYPRTNKQNQKKKRKVEPPTPQEPGPAKVAVTSSSSSSSSSSSIPSAEKVPTTKSTLWQEEMRTKDQPDGSSLSPAQSPSQSQPPAASSLREPGLESKEEESAMSSDRMDCGRKVRVESGYFSLEKTKQDLKAEEQQLPPPLSPPSPSTPNHRRSQVIEKFEALDIEKAEHMETNAVGPSPSSDTRQGRSEKRAFPRKRDFTNEAPPAPLPDASASPLSPHRRAKSLDRRSTEPSVTPDLLNFKKGWLTKQYEDGQWKKHWFVLADQSLRYYRDSVAEEAADLDGEIDLSACYDVTEYPVQRNYGFQIHTKEGEFTLSAMTSGIRRNWIQTIMKHVHPTTAPDVTSSLPEEKNKSSCSFETCPRPTEKQEAELGEPDPEQKRSRARERRREGRSKTFDWAEFRPIQQALAQERVGGVGPADTHEPLRPEAEPGELERERARRREERRKRFGMLDATDGPGTEDAALRMEVDRSPGLPMSDLKTHNVHVEIEQRWHQVETTPLREEKQVPIAPVHLSSEDGGDRLSTHELTSLLEKELEQSQKEASDLLEQNRLLQDQLRVALGREQSAREGYVLQATCERGFAAMEETHQKKIEDLQRQHQRELEKLREEKDRLLAEETAATISAIEAMKNAHREEMERELEKSQRSQISSVNSDVEALRRQYLEELQSVQRELEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITRLRTLLTGDGGGEATGSPLAQGKDAYELEVLLRVKESEIQYLKQEISSLKDELQTALRDKKYASDKYKDIYTELSIAKAKADCDISRLKEQLKAATEALGEKSPDSATVSGYDIMKSKSNPDFLKKDRSCVTRQLRNIRSKSVIEQVSWDT

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q6WCQ1 作为底物

Site PTM Type Enzyme
K5 Ubiquitination
T166 Phosphorylation
S181 Phosphorylation
S182 Phosphorylation
S183 Phosphorylation
S184 Phosphorylation
S192 Phosphorylation
T210 Phosphorylation
S217 Phosphorylation
S218 Phosphorylation
S220 Phosphorylation
S224 Phosphorylation
S226 Phosphorylation
S228 Phosphorylation
S265 Phosphorylation
Y267 Phosphorylation
S269 Phosphorylation
S289 Phosphorylation
S292 Phosphorylation
S294 Phosphorylation
T295 Phosphorylation
S301 Phosphorylation
K306 Ubiquitination
S326 Phosphorylation
S329 Phosphorylation
T348 Phosphorylation
S360 Phosphorylation
S362 Phosphorylation
S365 Phosphorylation
S372 Phosphorylation
S377 Phosphorylation
T378 Phosphorylation
T383 Phosphorylation
K390 Ubiquitination
K396 Ubiquitination
Y450 Phosphorylation
S493 Phosphorylation
S501 Phosphorylation
S504 Phosphorylation
K527 Ubiquitination
S540 Phosphorylation
T542 Phosphorylation
T602 Phosphorylation
S619 Phosphorylation
S625 Phosphorylation
T646 Phosphorylation
S662 Phosphorylation
S663 Phosphorylation
K681 Ubiquitination
K688 Ubiquitination
S691 Phosphorylation
S713 Phosphorylation
Y718 Phosphorylation
S800 Phosphorylation
S815 Phosphorylation
S891 Phosphorylation
Y915 Phosphorylation
K917 Ubiquitination
S921 Phosphorylation
Y945 Phosphorylation
T946 Phosphorylation
T970 Phosphorylation
K976 Ubiquitination
S977 Phosphorylation
S980 Phosphorylation
S984 Phosphorylation
Y986 Phosphorylation
K990 Methylation
S991 Phosphorylation
K992 Methylation
S993 Phosphorylation
S1014 Phosphorylation
S1016 Phosphorylation

研究背景

功能:

Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.

细胞定位:

Cytoplasm>Cytoskeleton.
Note: Colocalizes with F-actin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds F-actin through its N-terminus. Interacts with MYZAP (By similarity). Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.

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