产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4783, RRID:AB_2837137.
展开/折叠
LOK; Lymphocyte oriented kinase; Lymphocyte-oriented kinase; PRO2729; Serine threonine protein kinase 10; Serine/threonine-protein kinase 10; STK10; STK10_HUMAN;
抗原和靶标
Highly expressed in rapidly proliferating tissues (spleen, placenta, and peripheral blood leukocytes). Also expressed in brain, heart, skeletal muscle, colon, thymus, kidney, liver, small intestine and lung.
- O94804 STK10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAFANFRRILRLSTFEKRKSREYEHVRRDLDPNEVWEIVGELGDGAFGKVYKAKNKETGALAAAKVIETKSEEELEDYIVEIEILATCDHPYIVKLLGAYYHDGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQVVCRQMLEALNFLHSKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTPYWMAPEVVMCETMKDTPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLLEHPFVSSITSNKALRELVAEAKAEVMEEIEDGRDEGEEEDAVDAASTLENHTQNSSEVSPPSLNADKPLEESPSTPLAPSQSQDSVNEPCSQPSGDRSLQTTSPPVVAPGNENGLAVPVPLRKSRPVSMDARIQVAQEKQVAEQGGDLSPAANRSQKASQSRPNSSALETLGGEKLANGSLEPPAQAAPGPSKRDSDCSSLCTSESMDYGTNLSTDLSLNKEMGSLSIKDPKLYKKTLKRTRKFVVDGVEVSITTSKIISEDEKKDEEMRFLRRQELRELRLLQKEEHRNQTQLSNKHELQLEQMHKRFEQEINAKKKFFDTELENLERQQKQQVEKMEQDHAVRRREEARRIRLEQDRDYTRFQEQLKLMKKEVKNEVEKLPRQQRKESMKQKMEEHTQKKQLLDRDFVAKQKEDLELAMKRLTTDNRREICDKERECLMKKQELLRDREAALWEMEEHQLQERHQLVKQQLKDQYFLQRHELLRKHEKEREQMQRYNQRMIEQLKVRQQQEKARLPKIQRSEGKTRMAMYKKSLHINGGGSAAEQREKIKQFSQQEEKRQKSERLQQQQKHENQMRDMLAQCESNMSELQQLQNEKCHLLVEHETQKLKALDESHNQNLKEWRDKLRPRKKALEEDLNQKKREQEMFFKLSEEAECPNPSTPSKAAKFFPYSSADAS
翻译修饰 - O94804 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R11 | Methylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| T185 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| T391 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | O94804 (STK10) | Uniprot |
| T459 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| T530 | Phosphorylation | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| S679 | Phosphorylation | Uniprot | |
| T688 | Phosphorylation | Uniprot | |
| K690 | Acetylation | Uniprot | |
| K701 | Ubiquitination | Uniprot | |
| K759 | Ubiquitination | Uniprot | |
| K796 | Ubiquitination | Uniprot | |
| K823 | Ubiquitination | Uniprot | |
| S824 | Phosphorylation | Uniprot | |
| K841 | Ubiquitination | Uniprot | |
| S844 | Phosphorylation | Uniprot | |
| K849 | Ubiquitination | Uniprot | |
| S951 | Phosphorylation | Uniprot | |
| T952 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S954 | Phosphorylation | Uniprot | |
| Y962 | Phosphorylation | Uniprot | |
| S968 | Phosphorylation | Uniprot |
翻译修饰 - O94804 作为激酶
研究背景
Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.
Autophosphorylates following homodimerization, leading to activation of the protein.
Cell membrane>Peripheral membrane protein.
Highly expressed in rapidly proliferating tissues (spleen, placenta, and peripheral blood leukocytes). Also expressed in brain, heart, skeletal muscle, colon, thymus, kidney, liver, small intestine and lung.
Homodimer; homodimerization is required for activation segment autophosphorylation.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
研究领域
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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