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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4790, RRID:AB_2837144.
展开/折叠
DBK; PAK 1; PAK-1; PAK1; PKC1; PKN ALPHA; PKN; Pkn1; PKN1_HUMAN; PRK1; PRKCL1; Protease activated kinase 1; Protease-activated kinase 1; Protein kinase C like 1; Protein kinase C like PKN; Protein kinase C related kinase 1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase N1; Protein kinase PKN alpha; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine threonine kinase N; Serine threonine protein kinase N; Serine-threonine protein kinase N; Serine/threonine protein kinase N1; Serine/threonine-protein kinase N1;
抗原和靶标
Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
- Q16512 PKN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q16512 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| T63 | Phosphorylation | Uniprot | |
| T64 | Phosphorylation | Q16512 (PKN1) | Uniprot |
| S69 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| R357 | Methylation | Uniprot | |
| R366 | Methylation | Uniprot | |
| S372 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Q16512 (PKN1) | Uniprot |
| S379 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| K448 | Acetylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| T527 | Phosphorylation | Uniprot | |
| T529 | Phosphorylation | Uniprot | |
| T531 | Phosphorylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| T557 | Phosphorylation | Uniprot | |
| S559 | Phosphorylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| K565 | Ubiquitination | Uniprot | |
| S573 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| K607 | Ubiquitination | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| T611 | Phosphorylation | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| K664 | Ubiquitination | Uniprot | |
| K742 | Ubiquitination | Uniprot | |
| K763 | Ubiquitination | Uniprot | |
| Y768 | Phosphorylation | Uniprot | |
| T772 | Phosphorylation | Uniprot | |
| S773 | Phosphorylation | O15530 (PDPK1) | Uniprot |
| T774 | Phosphorylation | Q16512 (PKN1) , O15530 (PDPK1) | Uniprot |
| T778 | Phosphorylation | Uniprot | |
| R847 | Methylation | Uniprot | |
| S902 | Phosphorylation | Uniprot | |
| T914 | Phosphorylation | Uniprot | |
| S916 | Phosphorylation | Uniprot | |
| T925 | Phosphorylation | Uniprot |
翻译修饰 - Q16512 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O95863 (SNAI1) | S246 | Uniprot |
| P00533 (EGFR) | T678 | Uniprot |
| P04049 (RAF1) | S338 | Uniprot |
| P04049 (RAF1) | S339 | Uniprot |
| P10636-6 (MAPT) | T147 | Uniprot |
| P10636-2 (MAPT) | S156 | Uniprot |
| P10636-2 (MAPT) | S200 | Uniprot |
| P10636-8 (MAPT) | S214 | Uniprot |
| P10636-2 (MAPT) | S231 | Uniprot |
| P10636-8 (MAPT) | S258 | Uniprot |
| P10636-6 (MAPT) | S262 | Uniprot |
| P10636-2 (MAPT) | S263 | Uniprot |
| P10636-6 (MAPT) | S294 | Uniprot |
| P10636-8 (MAPT) | S320 | Uniprot |
| P10636-8 (MAPT) | S352 | Uniprot |
| P10636 (MAPT) | S637 | Uniprot |
| P18669 (PGAM1) | S23 | Uniprot |
| P18669 (PGAM1) | S118 | Uniprot |
| P29966 (MARCKS) | S159 | Uniprot |
| P29966 (MARCKS) | S163 | Uniprot |
| P29966 (MARCKS) | S170 | Uniprot |
| P30307 (CDC25C) | S216 | Uniprot |
| P30622 (CLIP1) | S312 | Uniprot |
| P36871 (PGM1) | T467 | Uniprot |
| Q12778 (FOXO1) | S256 | Uniprot |
| Q13077 (TRAF1) | S146 | Uniprot |
| Q16512-1 (PKN1) | T64 | Uniprot |
| Q16512-2 (PKN1) | T70 | Uniprot |
| Q16512-1 (PKN1) | S372 | Uniprot |
| Q16512-1 (PKN1) | S374 | Uniprot |
| Q16512 (PKN1) | S376 | Uniprot |
| Q16512-2 (PKN1) | S378 | Uniprot |
| Q16512-1 (PKN1) | S380 | Uniprot |
| Q16512-1 (PKN1) | T531 | Uniprot |
| Q16512-2 (PKN1) | T537 | Uniprot |
| Q16512-1 (PKN1) | S576 | Uniprot |
| Q16512-2 (PKN1) | S582 | Uniprot |
| Q16512-1 (PKN1) | S608 | Uniprot |
| Q16512-2 (PKN1) | S614 | Uniprot |
| Q16512 (PKN1) | T774 | Uniprot |
| Q92974 (ARHGEF2) | S886 | Uniprot |
| Q9UKV0 (HDAC9) | S253 | Uniprot |
| Q9UQL6 (HDAC5) | T292 | Uniprot |
研究背景
PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
Autophosphorylated; preferably on serine. Phosphorylated during mitosis.
Activated by limited proteolysis with trypsin.
(Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation.
Cytoplasm. Nucleus. Endosome. Cell membrane>Peripheral membrane protein. Cleavage furrow. Midbody.
Note: Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.
Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
Interacts with ZFAND6 (By similarity). Interacts with ANDR. Interacts with PRKCB. Interacts (via REM 1 and REM 2 repeats) with RAC1. Interacts (via REM 1 repeat) with RHOA. Interacts with RHOB. Interacts (via C-terminus) with PDPK1. Interacts with CCNT2; enhances MYOD1-dependent transcription. Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK.
(Microbial infection) Interacts (via the second REM repeat) with S.typhimurium E3 ubiquitin-protein ligase SspH1 (via the leucine-rich repeat region).
The C1 domain does not bind the diacylglycerol (DAG).
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
研究领域
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
文献引用
Application: WB Species: Rat Sample:
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