产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4799, RRID:AB_2837153.
展开/折叠
ACT; ACTBL_HUMAN; ACTBL2; actin like protein; actin, beta like 2; Beta-actin-like protein 2; DKFZp686D0972; kappa actin; Kappa-actin;
抗原和靶标
- Q562R1 ACTBL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTDNELSALVVDNGSGMCKAGFGGDDAPRAVFPSMIGRPRHQGVMVGMGQKDCYVGDEAQSKRGVLTLKYPIEHGVVTNWDDMEKIWYHTFYNELRVAPDEHPILLTEAPLNPKINREKMTQIMFEAFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHIVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYNFTTTAEREIVRDVKEKLCYVALDFEQEMVRAAASSSPERSYELPDGQVITIGNERFRCPEAIFQPSFLGIESSGIHETTFNSIMKCDVDIRKDLYANTVLSGGSTMYPGIADRMQKEIITLAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPPIVHRKCF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q562R1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| K51 | Acetylation | Uniprot | |
| K51 | Methylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| Y54 | Phosphorylation | Uniprot | |
| K62 | Acetylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K69 | Methylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| T107 | Phosphorylation | Uniprot | |
| T121 | Phosphorylation | Uniprot | |
| Y144 | Phosphorylation | Uniprot | |
| T161 | Phosphorylation | Uniprot | |
| Y170 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| Y189 | Phosphorylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| K192 | Methylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| C286 | S-Nitrosylation | Uniprot | |
| K292 | Sumoylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| Y295 | Phosphorylation | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| Y307 | Phosphorylation | Uniprot | |
| R313 | Methylation | Uniprot | |
| T320 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| K329 | Acetylation | Uniprot | |
| K329 | Sumoylation | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| K337 | Sumoylation | Uniprot | |
| S349 | Phosphorylation | Uniprot |
研究背景
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).
Monomethylation at Lys-85 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (By similarity).
Cytoplasm>Cytoskeleton.
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others (By similarity). Interacts with PFN1 and PFDN1.
Belongs to the actin family.
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