LHX1 Antibody - #DF4823

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产品: LHX1 抗体
货号: DF4823
描述: Rabbit polyclonal antibody to LHX1
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 44 KD; 45kD(Calculated).
蛋白号: P48742
RRID: AB_2837188

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货期: 当天发货

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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Zebrafish(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(91%), Xenopus(91%)
克隆:
Polyclonal
特异性:
LHX1 Antibody detects endogenous levels of total LHX1.
RRID:
AB_2837188
引用格式: Affinity Biosciences Cat# DF4823, RRID:AB_2837188.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

hLim-1; Homeo box protein Lim 1; Homeo box protein Lim1; Homeobox protein Lim 1; Homeobox protein Lim-1; Homeobox protein Lim1; LHX 1; LHX1; LHX1_HUMAN; LIM 1; LIM homeo box 1; LIM homeo box protein 1; LIM homeobox 1; LIM homeobox protein 1; LIM-1; LIM/homeobox protein Lhx 1; LIM/homeobox protein Lhx1; MGC126723; MGC138141;

抗原和靶标

免疫原:
Uniprot:

P48742(LHX1_HUMAN) >>Visit HPA database.

基因/基因ID:
LHX1(3975)
表达:
P48742 LHX1_HUMAN:

Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.

序列:
MVHCAGCKRPILDRFLLNVLDRAWHVKCVQCCECKCNLTEKCFSREGKLYCKNDFFRCFGTKCAGCAQGISPSDLVRRARSKVFHLNCFTCMMCNKQLSTGEELYIIDENKFVCKEDYLSNSSVAKENSLHSATTGSDPSLSPDSQDPSQDDAKDSESANVSDKEAGSNENDDQNLGAKRRGPRTTIKAKQLETLKAAFAATPKPTRHIREQLAQETGLNMRVIQVWFQNRRSKERRMKQLSALGARRHAFFRSPRRMRPLVDRLEPGELIPNGPFSFYGDYQSEYYGPGGNYDFFPQGPPSSQAQTPVDLPFVPSSGPSGTPLGGLEHPLPGHHPSSEAQRFTDILAHPPGDSPSPEPSLPGPLHSMSAEVFGPSPPFSSLSVNGGASYGNHLSHPPEMNEAAVW

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Xenopus
91
Chicken
91
Rabbit
91
Zebrafish
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P48742 作为底物

Site PTM Type Enzyme
K48 Sumoylation
S71 Phosphorylation
S162 Phosphorylation

研究背景

功能:

Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.

亚基结构:

Interacts with LDB1 via the tandem LIM domains.

蛋白家族:

The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox (By similarity).

文献引用

1). BUB1 drives the occurrence and development of bladder cancer by mediating the STAT3 signaling pathway. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2021 (PubMed: 34852826) [IF=11.3]
2). Degradation of Different Molecular Weight Fucoidans and Their Inhibition of TGF-β1 Induced Epithelial-Mesenchymal Transition in Mouse Renal Tubular Epithelial Cells. International Journal of Biological Macromolecules, 2020 (PubMed: 32057857) [IF=7.7]

Application: IF/ICC    Species: Mouse    Sample: MTEC cells

Fig. 7. The result of cell immunofluorescence assay after LHXs and TGF-β1 treated MTEC for 24 and 48 h. Representative images (3 visual fields for each tissue analyzed) of immunolabeling for Fn and nuclear staining with DAPI. Scale bar, 50 ~μm.
3). Long non-coding RNA LHX1-DT regulates cardiomyocyte differentiation through H2A.Z-mediated LHX1 transcriptional activation. iScience, 2023 (PubMed: 37942009)

Application: WB    Species: Human    Sample:

Figure 4 LHX1-DT enhances LHX1 expression to promote cardiac differentiation (A) Schematic representation of the LHX1-DT and LHX1 gene locus and their sequence conservation analysis from UCSC genome browser. Red and blue arrows indicate the direction of transcription of LHX1-DT and LHX1 respectively. (B) Genome browser track of the selected genomic regions around LHX1-DT and LHX1. The gray shadow rectangle showing the expression levels and ATAC signals of LHX1-DT and LHX1 at the four stages of cardiac development. (C) qRT-PCR analysis of LHX1-DT and LHX1 expression during cardiac differentiation, n = 4. (D) Localization of LHX1-DT and LHX1 in mesodermal cells derived from hESCs by FISH study. Scale bars, 20 μm, n = 4. (E) Western blot analysis of LHX1 in undifferentiated and in mesoderm differentiating cells with LHX1-DT knockdown. ∗∗p < 0.01, n = 3. (F) qRT-PCR analysis of LHX1 expression in differentiating hESCs 2 days following siRNA transfection of cardiac differentiation. ∗∗∗p < 0.001, n = 4. (G and H) qRT-PCR analysis of the expression levels of LHX1-DT and mesoderm markers (T and Mesp1) on Day 2 and cardiomyocyte markers (cTnT, α-actinin, NKX2.5, and MYL7) on Day 10 in LHX1-DT depleted hESCs. ∗∗∗p < 0.001, n = 4. (I) Immunostaining of cTnT (green) and α-actinin (red) in LHX1 knockdown and siNC cells on Day 10 of CM differentiation. Scale bar, 50 μm, n = 4. (J) Representative flow cytometry analysis of the percentage of cTnT-positive cardiomyocytes between siLHX1 and siNC group. ∗∗∗p < 0.001, n = 3. (K) qRT-PCR analysis of cardiac marker genes expression on Day 10 in LHX1-DT+/+, LHX1-DT−/−, and LHX1-DT−/− + LHX1 cells. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. LHX1-DT+/+, n = 4. ##p < 0.01, ###p < 0.001 vs. LHX1-DT−/−, n = 4. (L) Immunostaining of cTnT (green) and α-actinin (red) in LHX1-DT+/+, LHX1-DT−/−, and LHX1-DT−/− + LHX1 cells on Day 10 of CM differentiation. Scale bar, 50 μm. Scale bars, 50 μm, n = 4. (M) Representative flow cytometry analysis of the percentage of cTnT-positive cells in LHX1-DT+/+, LHX1-DT−/−, and LHX1-DT−/− + LHX1 cardiomyocytes. ∗∗∗p < 0.001 vs. LHX1-DT+/+, ###p < 0.001 vs. LHX1-DT−/−, n = 3. Data are presented as mean ± SEM.

Application: IF/ICC    Species: Human    Sample:

Figure 4 LHX1-DT enhances LHX1 expression to promote cardiac differentiation (A) Schematic representation of the LHX1-DT and LHX1 gene locus and their sequence conservation analysis from UCSC genome browser. Red and blue arrows indicate the direction of transcription of LHX1-DT and LHX1 respectively. (B) Genome browser track of the selected genomic regions around LHX1-DT and LHX1. The gray shadow rectangle showing the expression levels and ATAC signals of LHX1-DT and LHX1 at the four stages of cardiac development. (C) qRT-PCR analysis of LHX1-DT and LHX1 expression during cardiac differentiation, n = 4. (D) Localization of LHX1-DT and LHX1 in mesodermal cells derived from hESCs by FISH study. Scale bars, 20 μm, n = 4. (E) Western blot analysis of LHX1 in undifferentiated and in mesoderm differentiating cells with LHX1-DT knockdown. ∗∗p < 0.01, n = 3. (F) qRT-PCR analysis of LHX1 expression in differentiating hESCs 2 days following siRNA transfection of cardiac differentiation. ∗∗∗p < 0.001, n = 4. (G and H) qRT-PCR analysis of the expression levels of LHX1-DT and mesoderm markers (T and Mesp1) on Day 2 and cardiomyocyte markers (cTnT, α-actinin, NKX2.5, and MYL7) on Day 10 in LHX1-DT depleted hESCs. ∗∗∗p < 0.001, n = 4. (I) Immunostaining of cTnT (green) and α-actinin (red) in LHX1 knockdown and siNC cells on Day 10 of CM differentiation. Scale bar, 50 μm, n = 4. (J) Representative flow cytometry analysis of the percentage of cTnT-positive cardiomyocytes between siLHX1 and siNC group. ∗∗∗p < 0.001, n = 3. (K) qRT-PCR analysis of cardiac marker genes expression on Day 10 in LHX1-DT+/+, LHX1-DT−/−, and LHX1-DT−/− + LHX1 cells. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. LHX1-DT+/+, n = 4. ##p < 0.01, ###p < 0.001 vs. LHX1-DT−/−, n = 4. (L) Immunostaining of cTnT (green) and α-actinin (red) in LHX1-DT+/+, LHX1-DT−/−, and LHX1-DT−/− + LHX1 cells on Day 10 of CM differentiation. Scale bar, 50 μm. Scale bars, 50 μm, n = 4. (M) Representative flow cytometry analysis of the percentage of cTnT-positive cells in LHX1-DT+/+, LHX1-DT−/−, and LHX1-DT−/− + LHX1 cardiomyocytes. ∗∗∗p < 0.001 vs. LHX1-DT+/+, ###p < 0.001 vs. LHX1-DT−/−, n = 3. Data are presented as mean ± SEM.

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