RRM2B Antibody - #DF4826

(2)  
产品: RRM2B 抗体
货号: DF4826
描述: Rabbit polyclonal antibody to RRM2B
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Dog
分子量: 40 KD; 41kD(Calculated).
蛋白号: Q7LG56
RRID: AB_2837191

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
RRM2B Antibody detects endogenous levels of total RRM2B.
RRID:
AB_2837191
引用格式: Affinity Biosciences Cat# DF4826, RRID:AB_2837191.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp686M05248; MGC102856; MGC42116; MTDPS8A; MTDPS8B; p53 inducible ribonucleotide reductase small subunit 2 homolog; p53 inducible ribonucleotide reductase small subunit 2 like protein; P53 inducible ribonucleotide reductase small subunit 2 short form beta; p53 R2; p53-inducible ribonucleotide reductase small subunit 2-like protein; p53R2; Ribonucleoside diphosphate reductase M2 subunit B; Ribonucleoside-diphosphate reductase subunit M2 B; Ribonucleotide reductase M2 B (TP53 inducible); Ribonucleotide reductase M2 B; Ribonucleotide reductase small subunit like 2 p53 inducible; RIR2B_HUMAN; RRM 2B; RRM2B; TP53 inducible ribonucleotide reductase M2 B; TP53-inducible ribonucleotide reductase M2 B;

抗原和靶标

免疫原:
Uniprot:

Q7LG56(RIR2B_HUMAN) >>Visit HPA database.

基因/基因ID:
RRM2B(50484)
表达:
Q7LG56 RIR2B_HUMAN:

Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma.

序列:
MGDPERPEAAGLDQDERSSSDTNESEIKSNEEPLLRKSSRRFVIFPIQYPDIWKMYKQAQASFWTAEEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEARCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRKSTFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQYLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFSKVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Horse
77
Rabbit
75
Pig
69
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q7LG56 作为底物

Site PTM Type Enzyme
R17 Methylation
S20 Phosphorylation
K28 Ubiquitination
S72 Phosphorylation Q13315 (ATM)
K166 Ubiquitination
K167 Ubiquitination
K168 Ubiquitination
S225 Phosphorylation
K326 Acetylation
K326 Ubiquitination
S329 Phosphorylation
Y331 Phosphorylation

研究背景

功能:

Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.

细胞定位:

Cytoplasm. Nucleus.
Note: Translocates from cytoplasm to nucleus in response to DNA damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma.

亚基结构:

Heterotetramer with large (RRM1) subunit. Interacts with p53/TP53. Interacts with RRM1 in response to DNA damage.

蛋白家族:

Belongs to the ribonucleoside diphosphate reductase small chain family.

研究领域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

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