产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4867, RRID:AB_2837230.
展开/折叠
CD363; CHEDG 1; CHEDG1; D1S3362; ECGF 1; ECGF1; EDG 1; EDG1; endothelial differentiation G protein coupled receptor 1; Endothelial differentiation G-protein coupled receptor 1; Endothelial differentiation sphingolipid G protein coupled receptor 1; FLJ58121; G protein coupled sphingolipid receptor; g protein-coupled receptor edg-1; S1P receptor 1; S1P receptor Edg 1; S1P receptor Edg-1; S1P receptor Edg1; S1P(1) receptor; S1P1; s1pr1; S1PR1_HUMAN; sphingolipid g-protein-coupled receptor 1; Sphingosine 1 phosphate receptor Edg 1; Sphingosine 1 phosphate receptor EDG1; sphingosine 1- phosphate receptor 1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; Sphingosine 1phosphate receptor type 1 S1P1;
抗原和靶标
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
- P21453 S1PR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPTSVPLVKAHRSSVSDYVNYDIIVRHYNYTGKLNISADKENSIKLTSVVFILICCFIILENIFVLLTIWKTKKFHRPMYYFIGNLALSDLLAGVAYTANLLLSGATTYKLTPAQWFLREGSMFVALSASVFSLLAIAIERYITMLKMKLHNGSNNFRLFLLISACWVISLILGGLPIMGWNCISALSSCSTVLPLYHKHYILFCTTVFTLLLLSIVILYCRIYSLVRTRSRRLTFRKNISKASRSSEKSLALLKTVIIVLSVFIACWAPLFILLLLDVGCKVKTCDILFRAEYFLVLAVLNSGTNPIIYTLTNKEMRRAFIRIMSCCKCPSGDSAGKFKRPIIAGMEFSRSKSDNSSHPQKDEGDNPETIMSSGNVNSSS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P21453 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T4 | O-Glycosylation | Uniprot | |
| S5 | O-Glycosylation | Uniprot | |
| N30 | N-Glycosylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| Y143 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K330 | Acetylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot |
研究背景
G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis (By similarity). Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury.
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
Cell membrane>Multi-pass membrane protein. Endosome. Membrane raft.
Note: Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization.
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
Interacts with GNAI1 and GNAI3.
Belongs to the G-protein coupled receptor 1 family.
研究领域
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
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