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产品:	ADORA1 抗体
货号:	DF4905
描述:	Rabbit polyclonal antibody to ADORA1
应用:	WB
反应:	Human, Rat
预测:	Pig, Horse, Rabbit
分子量:	37 KD; 37kD(Calculated).
蛋白号:	P30542
RRID:	AB_2837258

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封闭肽

阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Rat

预测:

Pig(83%), Horse(92%), Rabbit(89%)

克隆:

Polyclonal

特异性:

ADORA1 Antibody detects endogenous levels of total ADORA1.

RRID:

AB_2837258
引用格式: Affinity Biosciences Cat# DF4905, RRID:AB_2837258.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

A1AR; A1R; AA1R; AA1R_HUMAN; Adenosine receptor A1; ADORA 1; ADORA 1; ADORA1; RDC 7; RDC7; Ri;

抗原和靶标

免疫原:

Uniprot:

P30542(AA1R_HUMAN) >>Visit HPA database.

基因/基因ID:

ADORA1(134)

序列:

P30542 AA1R_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPPSISAFQAAYIGIEVLIALVSVPGNVLVIWAVKVNQALRDATFCFIVSLAVADVAVGALVIPLAILINIGPQTYFHTCLMVACPVLILTQSSILALLAIAVDRYLRVKIPLRYKMVVTPRRAAVAIAGCWILSFVVGLTPMFGWNNLSAVERAWAANGSMGEPVIKCEFEKVISMEYMVYFNFFVWVLPPLLLMVLIYLEVFYLIRKQLNKKVSASSGDPQKYYGKELKIAKSLALILFLFALSWLPLHILNCITLFCPSCHKPSILTYIAIFLTHGNSAMNPIVYAFRIQKFRVTFLKIWNDHFRCQPAPPIDEDLPEERPDD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Horse

Rabbit

Pig

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P30542 作为底物

P30542

Site	PTM Type	Enzyme	Source
K214	Methylation		Uniprot
K224	Methylation		Uniprot

研究背景

P30542_AA1R_HUMAN

功能:

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

细胞定位:

Cell membrane>Multi-pass membrane protein.

蛋白家族:

Belongs to the G-protein coupled receptor 1 family.

研究领域

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

文献引用

1). Impact of Caffeine Consumption on Type 2 Diabetes-Induced Spatial Memory Impairment and Neurochemical Alterations in the Hippocampus. Frontiers in Neuroscience, 2019 (PubMed: 30686981) [IF=4.3]

Application: WB Species: rat Sample: hippocampus

Figure 6. |Density A1R and A2AR analysed by Western blot in nerve terminal-enriched membranes(A) and total membranes (B) from the hippocampus of GK and Wistar (W) rats, either receiving 1g/L caffeine (Caff) or tap water. Samples were loaded in SDS-PAGE gels at a protein concentration of 40 or 150 μg for A1R (n=6-8) and A2AR (n=2-3), respectively. Immunoreactivity was normalised to either α-tubulin or β-actin, and calculated as percentage of control in the same Western blot experiment. Symbols represent LSD test results after ANOVA with either significant diabetes effect or significant diabetes-caffeine interaction: *P<0.05, **P<0.01 for GK versus Wistar in the respective treatment group or as indicated.

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ADORA1 Antibody - #DF4905

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