产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4908, RRID:AB_2837261.
展开/折叠
7TM receptor; Acetylcholine receptor, muscarinic, 2; AChR; Acm2; ACM2_HUMAN; Cholinergic receptor muscarinic 2; Cholinergic receptor, muscarinic 2, cardiac; Cholinergic receptor, muscarinic 2, isoform a; Cholinergic receptor, muscarinic 2a; CHRM 2; CHRM2; chrm2a; CM2; FLJ43243; HM 2; HM2; M2; M2 muscarinic receptor; M2-mAChR; MGC120006; MGC120007; Muscarinic acetylcholine receptor M2; Muscarinic M2 receptor;
抗原和靶标
- P08172 ACM2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNNSTNSSNNSLALTSPYKTFEVVFIVLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGVRTVEDGECYIQFFSNAAVTFGTAIAAFYLPVIIMTVLYWHISRASKSRIKKDKKEPVANQDPVSPSLVQGRIVKPNNNNMPSSDDGLEHNKIQNGKAPRDPVTENCVQGEEKESSNDSTSVSAVASNMRDDEITQDENTVSTSLGHSKDENSKQTCIRIGTKTPKSDSCTPTNTTVEVVGSSGQNGDEKQNIVARKIVKMTKQPAKKKPPPSREKKVTRTILAILLAFIITWAPYNVMVLINTFCAPCIPNTVWTIGYWLCYINSTINPACYALCNATFKKTFKHLLMCHYKNIGATR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P08172 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y177 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | P25098 (GRK2) | Uniprot |
| S234 | Phosphorylation | P25098 (GRK2) | Uniprot |
| S250 | Phosphorylation | P25098 (GRK2) | Uniprot |
| S251 | Phosphorylation | P25098 (GRK2) | Uniprot |
| T271 | Phosphorylation | P25098 (GRK2) | Uniprot |
| S282 | Phosphorylation | P25098 (GRK2) | Uniprot |
| S283 | Phosphorylation | P25098 (GRK2) | Uniprot |
| S286 | Phosphorylation | P25098 (GRK2) | Uniprot |
| T287 | Phosphorylation | P25098 (GRK2) | Uniprot |
| S288 | Phosphorylation | P25098 (GRK2) | Uniprot |
| S290 | Phosphorylation | P25098 (GRK2) | Uniprot |
| S294 | Phosphorylation | P25098 (GRK2) | Uniprot |
| T302 | Phosphorylation | P25098 (GRK2) | Uniprot |
| T307 | Phosphorylation | P25098 (GRK2) | Uniprot |
| S309 | Phosphorylation | P25098 (GRK2) | Uniprot |
| T310 | Phosphorylation | P25098 (GRK2) | Uniprot |
| S311 | Phosphorylation | P25098 (GRK2) | Uniprot |
| T423 | Phosphorylation | Uniprot |
研究背景
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.
Phosphorylated in response to agonist treatment.
Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein.
Note: Phosphorylation in response to agonist binding promotes receptor internalization.
Interacts with ARRB1 and ARRB2. Interacts with RACK1; the interaction regulates CHRM2 internalization.
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.
研究领域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Cholinergic synapse.
限制条款
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