AGTR1 Antibody - #DF4910

Fig. 10. | Effect of XJEK on AT1R and NOX-4 levels in renal cortical tissues of MI rats. The concentration levels of Ang II type 1 receptor (AT1R) and NADPH Oxidase-4 (NOX-4) proteins in renal cortical tissues by Western blot analysis and commercial ELISA kits. (A, B and C) AT1R content in renal cortical tissues of rats for 2, 4 and 6wk post-MI by western blot, respectively (mean ± SEM, n = 4 in each group)

Fig. S3.|AT1R expression was analyzed via Western blot. AT1R expression increased 2.5-fold in response to Ang II infusion, which was slightly decreased by miR144-5p agomirs.

FIGURE 8 ELISA and Western Blot results of aortic tissue in each group of mice. (A) ET-1 ELISA results were shown. Both telmisartan and nifedipine treatments can effectively reduce ET-1 serum levels in the AD mice model. Telmisartan is slightly better than nifedipine. (B) IL-6 ELISA results were shown. Both telmisartan and nifedipine treatments can effectively reduce IL-6 serum levels in the AD mice model. Telmisartan is slightly better than nifedipine. (C) MMP 9 ELISA results were shown. Both telmisartan and nifedipine treatments can effectively reduce MMP 9 serum levels in the AD mice model. Telmisartan is slightly better than nifedipine. (D) Western Blot results of YAP and AT1R content in aortic tissue of each group were demonstrated. The contents of AT1R and p-YAP in the aortic tissue of AD group were significantly increased, and the total content of YAP was decreased. Telmisartan treatment can effectively alleviate this phenomenon, and nifedipine cannot affect the expression of AT1R and YAP. (n = 3, mean and S.D., t-test, *P < 0.01 compared with control group; **P < 0.05 compared with the control group; ∧∧P < 0.05 compared with the AD group; ##P < 0.05 compared with the AD + telmisartan group).

Figure 3. |Ang II promotes AT1R expression and YAP phosphorylation (Ser127), and treatment with telmisartan or transfection with AT1R siRNA reverses this effect. Grouping: Control group, HAECs only; group 1, HAECs with Ang II (1 µM) treatment; group 2, HAECs with Ang II (1 µM) and siRNA NC transfection; group 3, HAECs with Ang II (1 µM) and AT1R siRNA transfection; group 4, HAECs with Ang II (1 µM) and 1 µl DMSO; and group 5, HAECs with Ang II (1 µM) and the ARB 1 µl (20 mM) telmisartan treatment. (A) Western blot analysis of sets of six independent lysates from HAECs that were untreated, treated with Ang II, treated with Ang II and siRNA NC, treated with Ang II and AT1R siRNA, treated with Ang II and DMSO, or treated with Ang II and telmisartan. Treatment with Ang II upregulated AT1R and p‑YAP, and this effect was alleviated by transfection with an AT1R siRNA or treatment with telmisartan.