EDNRA Antibody - #DF4923

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产品: EDNRA 抗体
货号: DF4923
描述: Rabbit polyclonal antibody to EDNRA
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 48 KD; 49kD(Calculated).
蛋白号: P25101
RRID: AB_2837276

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(100%)
克隆:
Polyclonal
特异性:
EDNRA Antibody detects endogenous levels of total EDNRA.
RRID:
AB_2837276
引用格式: Affinity Biosciences Cat# DF4923, RRID:AB_2837276.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Ednra; EDNRA_HUMAN; Endothelin 1 receptor precursor; Endothelin 1 specific receptor; Endothelin A receptor; Endothelin ET A receptor; Endothelin receptor ET1 specific type; Endothelin receptor subtype A; Endothelin receptor type A; Endothelin-1 receptor; ET A; ET-A; ETA; ETA R; ETA-R; ETAR; ETRA; G protein coupled receptor; hET AR; hET-AR; MFDA;

抗原和靶标

免疫原:
Uniprot:

P25101(EDNRA_HUMAN) >>Visit HPA database.

基因/基因ID:
EDNRA(1909)
表达:
P25101 EDNRA_HUMAN:

Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.

序列:
METLCLRASFWLALVGCVISDNPERYSTNLSNHVDDFTTFRGTELSFLVTTHQPTNLVLPSNGSMHNYCPQQTKITSAFKYINTVISCTIFIVGMVGNATLLRIIYQNKCMRNGPNALIASLALGDLIYVVIDLPINVFKLLAGRWPFDHNDFGVFLCKLFPFLQKSSVGITVLNLCALSVDRYRAVASWSRVQGIGIPLVTAIEIVSIWILSFILAIPEAIGFVMVPFEYRGEQHKTCMLNATSKFMEFYQDVKDWWLFGFYFCMPLVCTAIFYTLMTCEMLNRRNGSLRIALSEHLKQRREVAKTVFCLVVIFALCWFPLHLSRILKKTVYNEMDKNRCELLSFLLLMDYIGINLATMNSCINPIALYFVSKKFKNCFQSCLCCCCYQSKSLMTSVPMNGTSIQWKNHDQNNHNTDRSSHKDSMN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Chicken
100
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Rabbit
92
Xenopus
64
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P25101 作为底物

Site PTM Type Enzyme
Y184 Phosphorylation
Y263 Phosphorylation
Y275 Phosphorylation
S289 Phosphorylation
S295 Phosphorylation
Y333 Phosphorylation
K338 Acetylation
S382 Phosphorylation
Y389 Phosphorylation
S391 Phosphorylation
S393 Phosphorylation
T396 Phosphorylation
S397 Phosphorylation
T403 Phosphorylation
S404 Phosphorylation
T417 Phosphorylation
S420 Phosphorylation
S421 Phosphorylation
S425 Phosphorylation

研究背景

功能:

Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.

亚基结构:

Interacts with HDAC7 and KAT5.

蛋白家族:

Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily.

研究领域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Endocrine system > Renin secretion.

文献引用

1). Comprehensive analysis of expression signature and immune microenvironment signature of biomarker Endothelin Receptor Type A in stomach adenocarcinoma. Journal of Cancer, 2023 (PubMed: 35517422) [IF=3.9]

Application: IHC    Species: Human    Sample: STAD tissue

Figure 3 The preliminary immunohistochemistry analysis and clinical analysis of EDNRA in STAD. (A) Representative Immunohistochemistry image of EDNRA and subcellular staining localization in STAD and adjacent non-tumor tissue specimens. (B) The expression of EDNRA in STAD tissue was higher than adjacent non-tumor tissue (P
2). Deleterious effect in endothelin receptor–mediated coronary artery smooth muscle contractility in high-salt diet rats. Nutrition, Metabolism and Cardiovascular Diseases, 2023 (PubMed: 36404239) [IF=3.9]

Application: IHC    Species: Rat    Sample:

Figure 4Expression levels of ETA, ETB, STIM1, Orai1 in coronary arteries derived from 4% high-salt diet rats vs. controls. Western blot images (A–D) and summary data (E–H) of endothelial receptors ETA and ETB as well as of STIM1 and Orai1 in fresh isolated coronary arteries derived from rats fed a regular diet (control) and rats fed a high-salt diet. Protein expression levels were normalized to β-tubulin. Immunostaining results of ETA, ETB in segments of coronary arteries derived from control (I (a–b)) and high-salt diet (I (d–e)) rats (three rats in each group). The values are shown as mean ± SEM; n = 3–7 rats. ∗P < 0.05, ∗∗P < 0.01 compared with controls.

Application: WB    Species: Rat    Sample:

Figure 4Expression levels of ETA, ETB, STIM1, Orai1 in coronary arteries derived from 4% high-salt diet rats vs. controls. Western blot images (A–D) and summary data (E–H) of endothelial receptors ETA and ETB as well as of STIM1 and Orai1 in fresh isolated coronary arteries derived from rats fed a regular diet (control) and rats fed a high-salt diet. Protein expression levels were normalized to β-tubulin. Immunostaining results of ETA, ETB in segments of coronary arteries derived from control (I (a–b)) and high-salt diet (I (d–e)) rats (three rats in each group). The values are shown as mean ± SEM; n = 3–7 rats. ∗P < 0.05, ∗∗P < 0.01 compared with controls.

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