(1)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4980, RRID:AB_2837329.
展开/折叠
BPHS, mouse, homolog of; H1 R; H1R; HH1R; HisH1; Histamine H(1) receptor; Histamine H1 receptor; Histamine receptor H1; Histamine receptor subclass H1; Hrh1; HRH1_HUMAN;
抗原和靶标
- P35367 HRH1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLPNSSCLLEDKMCEGNKTTMASPQLMPLVVVLSTICLVTVGLNLLVLYAVRSERKLHTVGNLYIVSLSVADLIVGAVVMPMNILYLLMSKWSLGRPLCLFWLSMDYVASTASIFSVFILCIDRYRSVQQPLRYLKYRTKTRASATILGAWFLSFLWVIPILGWNHFMQQTSVRREDKCETDFYDVTWFKVMTAIINFYLPTLLMLWFYAKIYKAVRQHCQHRELINRSLPSFSEIKLRPENPKGDAKKPGKESPWEVLKRKPKDAGGGSVLKSPSQTPKEMKSPVVFSQEDDREVDKLYCFPLDIVHMQAAAEGSSRDYVAVNRSHGQLKTDEQGLNTHGASEISEDQMLGDSQSFSRTDSDTTTETAPGKGKLRSGSNTGLDYIKFTWKRLRSHSRQYVSGLHMNRERKAAKQLGFIMAAFILCWIPYFIFFMVIAFCKNCCNEHLHMFTIWLGYINSTLNPLIYPLCNENFKKTFKRILHIRS
翻译修饰 - P35367 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S68 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| T140 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| T142 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| S230 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| Y386 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Q9UQM7 (CAMK2A) , P17612 (PRKACA) , Q13976 (PRKG1) , P17252 (PRKCA) | Uniprot |
| S398 | Phosphorylation | Q13976 (PRKG1) , P17252 (PRKCA) , Q9UQM7 (CAMK2A) , P25098 (GRK2) , P17612 (PRKACA) | Uniprot |
| Y401 | Phosphorylation | Uniprot | |
| T478 | Phosphorylation | P17252 (PRKCA) | Uniprot |
研究背景
In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.
Phosphorylation at sites in the second and third cytoplasmic loops independently contribute to agonist-induced receptor downregulation.
Cell membrane>Multi-pass membrane protein.
Belongs to the G-protein coupled receptor 1 family.
研究领域
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
文献引用
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.